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pFH2.4_mCherry-MMLV_Psi

Plasmid

#205522

Purpose

Express mCherry-Psi (export tag consisting of MMLV Psi)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pwtCas9-bacteria

Plasmid

#44250

Purpose

aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

wild-type Cas9

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Tags

mCherry

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDonr201_PARG WT

Plasmid

#240315

Purpose

Entry vector for Gateway with PARG

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARG (PARG Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-L10A-Halo

Plasmid

#247300

Purpose

Mammalian expression of the large ribosomal submit protein L10A tagged with HaloTag; for single-molecule tracking of ribosomes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

pUBC-L10A-Halo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE47

Plasmid

#90385

Purpose

IFNB1 - Interferon beta gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IFNB1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHK346

Plasmid

#235477

Purpose

Inducible gene VIII by Pbad promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHK336

Plasmid

#235476

Purpose

Inducible gene VIII by Plux promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.2

Plasmid

#235461

Purpose

Message phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA2

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSV Sport RXR alpha

Plasmid

#8882

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

RXR alpha (RXRA Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC2

Plasmid

#22975

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC2 (CRTC2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pGK415-rtetTAK8N, L131L

Plasmid

#165969

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-zif268

Plasmid

#18045

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

zif268

Expression

Bacterial

Mutation

Zif268 is fused to the omega subunit of RNA polym…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO mKate2

Plasmid

#68441

Purpose

TOPO construct expressing mKate2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKate2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE12

Plasmid

#90347

Purpose

FXR - Farnesoid X receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

FXR

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP wild type

Plasmid

#174075

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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