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Flag-FOXF1

Plasmid

#153122

Purpose

Expression of Flag tagged human FOXF1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXF1 (FOXF1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Sp100B-C3

Plasmid

#134553

Purpose

Expresses YFP-Sp100B fuson protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100B

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC52A3

Plasmid

#132181

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A3 (C20orf54 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGAN201

Plasmid

#129204

Purpose

yTRAP sensor of [RNQ+], detects aggregation of Rnq1

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

RNQ1 (RNQ1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP1_G1

Plasmid

#127114

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-E2-Crimson

Plasmid

#111258

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

PpsbA

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS252

Plasmid

#73720

Purpose

SapTrap GFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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SBP-EGFP-Ecadherin_puromycin

Plasmid

#65292

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-23P639

Plasmid

#51388

Depositor

Narry Kim

Article

Lee et al EMBO J. 2004 Oct 13;23(20):4051-60. Epub

Insert

miR-23a promoter region (MIR23A Human)

Use

Luciferase

Tags

Luciferase

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
p2Bac.HA.p52

Plasmid

#39219

Depositor

Aziz Sancar

Insert

p52 (GTF2H4 Human)

Tags

HA

Expression

Insect

Promoter

Pp10

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pcihygrosynDAT

Plasmid

#19992

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

synDAT (SLC6A3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pGL-p27UTR

Plasmid

#20881

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p27 3'UTR (Cdkn1b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a2

Plasmid

#15748

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA-RAD52-WT

Plasmid

#235676

Purpose

Expresses HA-tagged wild type human RAD52 in mammalian cells.

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Insert

RAD52 (RAD52 Human)

Tags

HA

Expression

Mammalian

Mutation

wild type

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2554

Plasmid

#144129

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

AR (AR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1818

Plasmid

#142860

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

UHRF1 (UHRF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-sNRR-T2A-BFP

Plasmid

#194930

Purpose

LV construct encoding sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMesh1

Plasmid

#175594

Purpose

Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Article

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Mesh1 (Mesh1 Fly)

Expression

Bacterial

Mutation

Codon optimized for translation in E.coli. AGGAGG…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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