Skip to main content

More Filters

We narrowed to 23,118 results for: Sis

Showing: 2601 - 2650 of 23118 results
  1. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pVZ_PcoaT_SQE1

    Plasmid
    #103860
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Insert
    squalene epoxidase 1 (XF1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized. 2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pMRX-IP-muGFP-hATG2A mLIRΔWIR

    Plasmid
    #215508
    Purpose
    Expresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.
    Depositor
    Insert
    Autophagy related 2A (ATG2A Human)
    Use
    Retroviral
    Tags
    muGFP
    Expression
    Mammalian
    Mutation
    P656R (natural variant with no functional relevan…
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pVZ_PcoaT_SQE1_MRN1

    Plasmid
    #103858
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Insert
    marneral synthase 1 (MRN1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pVZ_PcoaT_SQE1_CAS1

    Plasmid
    #103856
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Insert
    cycloartenol synthase 1 (CAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pLenti-EML4-ALK variant 3a I1171N

    Plasmid
    #183832
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells
    Depositor
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid
    #183833
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells
    Depositor
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N/D1203N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pLlacO1-DP6

    Plasmid
    #242138
    Purpose
    AraC compatible Mutagenesis Circuit (drift plasmid)
    Depositor
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. PACE_AP_Pos_pBADProm

    Plasmid
    #242139
    Purpose
    pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution
    Depositor
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. PACE_AP_GIII_Titrate

    Plasmid
    #242140
    Purpose
    PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution
    Depositor
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. PACE_AP_Neg_pBADProm

    Plasmid
    #242141
    Purpose
    pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution
    Depositor
    Insert
    Gene III Negative
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  23. pSB-Bleo STAT3

    Plasmid
    #139184
    Purpose
    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
    Depositor
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    STAT3
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. AtFRD3

    Plasmid
    #117086
    Purpose
    protein expression in yeast
    Depositor
    Insert
    AtFRD3
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  25. AtAUX1

    Plasmid
    #117087
    Purpose
    protein expression in yeast
    Depositor
    Insert
    AtAUX1
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. SLAC1-FL

    Plasmid
    #117100
    Purpose
    protein expression in yeast
    Depositor
    Insert
    SLAC1-FL
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. pLVU/myc

    Plasmid
    #24179
    Depositor
    Insert
    Chloramphenicol resistance gene (CmR) - ccdB gene
    Use
    Lentiviral; Gateway
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only
  28. PLJR965_Rv3297

    Plasmid
    #241235
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3297) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv3297 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  29. PLJR965_Rv2976c

    Plasmid
    #241234
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2976c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv2976c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  30. PLJR965_Rv2464c

    Plasmid
    #241232
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv2464c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  31. PLJR965_Rv1638

    Plasmid
    #241231
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1638) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv1638 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  32. PLJR965_Rv1259

    Plasmid
    #241228
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1259) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv1259 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  33. PLJR965_Rv1020

    Plasmid
    #241227
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1020) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv1020 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  34. PLJR965_Rv0949

    Plasmid
    #241226
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv0949) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv0949 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  35. PLJR965_Rv1633

    Plasmid
    #241230
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1633) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv1633 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  36. PLJR965_Rv3589

    Plasmid
    #241236
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3589) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv3589 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  37. PLJR965_Rv2924c

    Plasmid
    #241233
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2924c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv2924c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  38. PLJR965_Rv1420

    Plasmid
    #241229
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1420) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Insert
    Rv1420 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  39. AtIRT1

    Plasmid
    #117884
    Purpose
    protein expression
    Depositor
    Insert
    AtIRT1 (IRT1 Mustard Weed)
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  40. AtPIN2

    Plasmid
    #117089
    Purpose
    protein expression in yeast
    Depositor
    Insert
    AtPIN2
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 2601 - 2650 of 23118 results