We narrowed to 13,730 results for: TIM
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Plasmid#162245PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-EGFP-eMagA
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZR069_CROPseq-sgPTPRC-CRISPRi
Plasmid#180271PurposeLentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRiDepositorInsertPTPRC (CD45) sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
eMagB-iRFP-Mito
Plasmid#162250PurposeBlue-light dependent heterodimerization systemDepositorInserteMagB-iRFP-OMP25
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)
Plasmid#191451PurposeVisualizing endogenous & active Cdc42DepositorInsertN-WASP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9
Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag7 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-VPS4A
Plasmid#115334Purposeexpresses mCherry-VPS4A in mammalian cellsDepositorInsertVPS4A (VPS4A Human)
UseRetroviralTagsmCherryExpressionMammalianMutationsilent mutation in position 4050 C to TPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-hNKD2
Plasmid#174328Purposeencodes human NKD2 cDNA with a C-terminal GFP-fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra1
Plasmid#179474PurposeExpresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cellsDepositorInsertsElectra1
mScarlet-I
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163043PurposeQuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-mCherry-eMagA
Plasmid#162248PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-mCherry-eMagA
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
eMagA-mCherry-Mito
Plasmid#162251PurposeBlue-light dependent heterodimerization systemDepositorInserteMagA-mCherry-OMP25
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNubWtXgate
Plasmid#105079PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneUseTagsNubIExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM3
Plasmid#154168PurposeEmpty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
LT3REVIR
Plasmid#111176PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5
Plasmid#104841Purposeb2AR-SPARK TF componentDepositorInsertb2AR-eLOV-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKeratin-Electra2
Plasmid#184928PurposeKeratin labeling with blue fluorescent protein Electra2DepositorInsertKeratin-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_112
Plasmid#136475PurposeLentiviral expression of AsCas12aDepositorInsertAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.336
Plasmid#108528PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.DepositorInsertLacIAM + PTac-YFP
UseTagsExpressionBacterialMutationLacI Q18M A47V F161YPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChR2(H134R)-GFP
Plasmid#58880PurposeAAV expression of ChR2(H134R)-GFP under the Syn promoterDepositorHas ServiceAAV Retrograde and AAV8InsertChR2(H134R)-GFP
UseAAVTagsGFPExpressionMammalianMutationH134RPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pElectra2-LaminA
Plasmid#179483PurposeNuclear envelope labeling with blue fluorescent protein Electra2DepositorInsertElectra2-LaminA
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DENV2-NS2B3_V5
Plasmid#115906PurposeMammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tagDepositorInsertDENV2 NS2B3 protease
UseTagsV5 tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-EYFP
Plasmid#20940PurposeMammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha WT
Plasmid#16390DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
3NES-CS(G)-tTA-CS(G)-3NES
Plasmid#137836PurposeSoluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequencesDepositorInsert3NES-CD(G)-tTA-CS(G)-3NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.847
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
UseTagsExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163072PurposeEntry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_888
Plasmid#201165PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD2-dU6:2gRNA
Plasmid#49409Purposeexpression of gRNA under control of the Drosophila U6:2 promoterDepositorInsertdU6-2:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-2Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
UseTagsExpressionBacterialMutationTtgR: R130H A144T A148VPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
flex-ChrimsonR-EYFP-Kv
Plasmid#135319PurposeCre-dependent expression of soma-targeted ChrimsonRDepositorInsertChrimsonR
UseAAVTagsEYFPExpressionMutationPromotersynapsinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChR2-mRuby2-ST
Plasmid#109125PurposeCation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChR2-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CIB-GFP
Plasmid#117242PurposeLight activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCIBN
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENO1-dTom-NATr
Plasmid#52208PurposeConstitutive expression of Candida codon-optimized dTomato with NAT selectable markerDepositorInsertdTomato
UseTagsExpressionBacterial and YeastMutationcodon optimized for Candida speciesPromoterENO1Available sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC64422
Plasmid#191768PurposeCRISPR mutagenesis in Medicago truncatulaDepositorInsertSpCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mCherry
Plasmid#169411PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherryDepositorInsertspLeft
mCherry
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-EGFP
Plasmid#53744PurposeContains human Histone H2B fused to EGFP in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsEGFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJune 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc double blue
Plasmid#170050PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blueDepositorInsertsmTagBFP2
CO BFP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T-VAPB(1- 218)- eMagB
Plasmid#162255PurposeBlue-light dependent heterodimerization systemDepositorInsertTagRFP-T-VAPB(1- 218)- eMagB
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
UseTagsExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2ox
Plasmid#124219PurposeCytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2oxDepositorInsertmNG-sfTq2ox
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Fd32
Plasmid#163964PurposeExpression of TPMV F glycoprotein in mammalian cellsDepositorInsertTPMV F glycoprotein
UseTagsExpressionMammalianMutationLacking 32 amino acids at C-terminusPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only