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ACE2(-1119)-luc

Plasmid

#31110

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -1119 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCAT203

Plasmid

#134880

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-GCaMP2

Plasmid

#18927

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2

Tags

His

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Expression

Plant

Promoter

U6 (Arabidopis)

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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p6592 MSCV-IP N-HAonly WWP2

Plasmid

#34905

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WWP2 (WWP2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116956

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID pRetrox

Plasmid

#155203

Purpose

For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

Depositor

Kyle Roux

Article

May et al Cells. 2020 Apr 25;9(5). pii: cells90510

Insert

3xHA-TurboID

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Promoter

tight TRE promotor

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Green Lindoblum

Plasmid

#163113

Purpose

Expresses biosensor for lactate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Lindolum

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD2

Plasmid

#21401

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN1 (EGLN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pOPINE

Plasmid

#26043

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His-lacZ-KHHHHHH

Tags

KHHHHHH and MAHHHHHHSSG

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pSO2002

Plasmid

#25842

Depositor

Aziz Sancar

Article

Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

Insert

human Cryptochrome 2 (CRY2 Human)

Tags

flag, his, and myc

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc2

Plasmid

#74050

Purpose

retroviral expression plasmid for human NFATc2/C

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 Human)

Use

Retroviral

Tags

no tag

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Luc2-P2A-copGFP

Plasmid

#72485

Purpose

Express Luc2 and copGFP

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-copGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 HA Sam68 WT

Plasmid

#17690

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-dCasRx-ALKBH5-PURO

Plasmid

#175582

Purpose

Expresses dCasRx-ALKBH5 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-ALKBH5

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RfxCas13d R239A/H244A/R858A/H863A

Promoter

pMSCV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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