Addgene

We narrowed to 2,312 results for: crispr system

Showing: 1041 - 1060 of 2312 results

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pGFP-PacI

Plasmid

#170100

Purpose

deGFP reporter plasmid with unique PacI restriction enzyme recognition site

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-ATAAC

Plasmid

#170095

Purpose

deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAS1sg

Plasmid

#154341

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAS1 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAS2sg

Plasmid

#154342

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAS2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CANTsg

Plasmid

#154343

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CANT sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAPsg

Plasmid

#154344

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAP sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pActin5c-CD-dCas9m4-UDI-attB

Plasmid

#104879

Purpose

To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9

Depositor

Christopher Potter

Insert

CD-dCas9(m4)-UDI

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-NIrD

Plasmid

#65010

Purpose

NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

Depositor

Wensheng Wei

Article

Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

Inserts

rtTA-MAVS(C)
2A-linked delta-TK

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Tight-TRE

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas3

Plasmid

#178881

Purpose

Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas3c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-SaCas9-pU6-sgRNA

Plasmid

#159173

Purpose

Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

SaCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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