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pX330A-Blimp1-L1-#1

Plasmid

#171503

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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E42_control_NGFR

Plasmid

#189800

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Article

Hosokawa et al Nat Immunol. 2018 Dec;19(12):1427-1440.

Insert

Luciferase gRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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1056F

Plasmid

#183134

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[tra]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PSMB5

Plasmid

#183317

Purpose

All-in-One CRISPRko system with a guide RNA that targets PSMB5 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PSMB5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_KDR

Plasmid

#183305

Purpose

All-in-One CRISPRko system with a guide RNA that targets KDR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

KDR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Mpp2 KI

Plasmid

#183434

Purpose

CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6.3

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2706

Plasmid

#175080

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 RC mutant

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Use

Tags

Expression

Mammalian

Mutation

RC mutant; sequence mutation: GCGGCCGCAGCGCA

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3971

Plasmid

#177736

Purpose

Prgef-1 GCaMP6::3xNLS::mNeptune

Depositor

Mei Zhen

Article

Susoy et al Cell. 2021 Sep 30;184(20):5122-5137.e17.

Insert

GCaMP

Use

Tags

mNeptune

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Dec. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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BII-Sh-PnGW-CMV-Luc

Plasmid

#133367

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W-Luc

Plasmid

#133362

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Use

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-Luc

Plasmid

#133360

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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LEP-shTADA1.1226

Plasmid

#105567

Purpose

retrovirally express TADA1 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5T

Plasmid

#62264

Purpose

expression of A5T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5NT

Plasmid

#62263

Purpose

expression of A5NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4T

Plasmid

#62262

Purpose

expression of A4T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1NT

Plasmid

#62255

Purpose

expression of A1NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1NT

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

TaU3

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJYS3_ΔcrtYf

Plasmid

#85542

Purpose

Constitutive transcription of FnCpf1 and crRNA of crtYf

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and Human U6

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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