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Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMP-NR2F1_2
Plasmid#36363DepositorInsertNR2F1 (NR2F1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB2801
Plasmid#63656PurposepCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD8
Plasmid#112820PurposepSMF2 with 8 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD10
Plasmid#112821PurposepSMF2 with 10 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMP-NR2F1_3
Plasmid#36364DepositorInsertNR2F1 (NR2F1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB2991
Plasmid#63643PurposepCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-Kdrl-3p-FokI-KK
Plasmid#35685DepositorInsertKdrl-3p-FokI-KK
UseMrna productionTagsHAExpressionMutationPromoterAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-Kdrl-5p-FokI-EL
Plasmid#35684DepositorInsertKdrl-5p-FokI-EL
UseMrna productionTagsFLAG and FlagExpressionMutationPromoterAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-NR2F1_1
Plasmid#36362DepositorInsertNR2F1 (NR2F1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
p072 pCS xGATA4 + xTRPS1(C119)
Plasmid#11043DepositorUseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationFusion construct of XGATA4 (amino acids 1-352) wi…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p300 KAT LIC 1M
Plasmid#233587PurposeBacterial expression of p300 KAT enzymeDepositorInsertp300 KAT (EP300 Human)
UseTags6xHis MBPExpressionBacterialMutationPromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-dgat1a in situ probe
Plasmid#232137Purposezebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertdgat1a (dgat1a Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-mogat2 in situ probe
Plasmid#232139Purposezebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat2 (mogat2 Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKM 1996
Plasmid#217825PurposeExpresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.DepositorInsertSA-phr1
UseTagsMaltose-binding proteinExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shWT1_A
Plasmid#217636PurposeDox inducible shRNA targeting WT-1DepositorInsertshWT1_A (WT1 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shWT1_B
Plasmid#217637PurposeDox inducible shRNA targeting WT-1DepositorInsertshWT1_B (WT1 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only