We narrowed to 13,582 results for: sequence
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pR023_PIE-PUM2
Plasmid#204819PurposeExpression PIE-PUM2 fusion protein in mammalian cellsDepositorInsertPumilio 2 (PUM2 Human)
TagsHA-Flag-rApobec1 and huADAR2ddExpressionMammalianPromoterchicken β-actin promoterAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-BlastR
Plasmid#196970PurposeLentiviral acceptor vector for pegRNAs with blasticidin resistanceDepositorTypeEmpty backboneUseCRISPRPromoterhU6Available SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript-humanAPOBEC1
Plasmid#134606PurposePlasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activityDepositorArticleAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-TagRFPT.zf1
Plasmid#61390PurposemRNA synthesis of TagRFPT codon modified for zebrafishDepositorInsertTagRFPT.zf1
UseZebrafish expressionPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NfsB.zf1
Plasmid#61387PurposemRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishDepositorInsertnfsB.zf1
UseZebrafish expressionMutationE28GPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IE1
Plasmid#52894Purposebaculovirus IE-1 promoter driving firefly luciferaseDepositorInsertIE1 promoter
UseLuciferasePromoterbaculovirus IE1Available SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-c-fos-iRFP-B3-repRNA-A
Plasmid#159926PurposeTimestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.DepositorInsertiRFP-Timestamp A
ExpressionMammalianAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pENTR2B-dCas9-KRAB
Plasmid#115547PurposeEntry cassette for dCas9-KRABDepositorInsertdCas9-KRAB
TagsHAExpressionBacterial and MammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
1.6 kB VEGF-luc
Plasmid#29667DepositorInsert1.6 kB VEGF
UseLuciferaseAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only
