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FRB-GFP-Gab1(Y628F/Y660F)

Plasmid

#188658

Purpose

FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 1 (Gab1 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y628F and Y660F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-DDX54-FLAG/HA

Plasmid

#97062

Purpose

Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Expression

Mammalian

Promoter

CAGGS

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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PB-Neo-TetO-Cdk12_Untagged

Plasmid

#127178

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Promoter

TetO Promoter

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo/Cre

Plasmid

#67594

Purpose

Expresses an Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgNeo

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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1017 pcDNA3 Myr Akt1 deltaPH

Plasmid

#9009

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 dPH (AKT1 Human)

Tags

myr (MGSSKSKPKDPSQR)

Expression

Mammalian

Mutation

delta PH domain (aa1-130)

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MED19

Plasmid

#15420

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab2(Y604F/Y634F)

Plasmid

#188659

Purpose

FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 2 (Gab2 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y604F and Y634F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Gnaq

Plasmid

#170298

Purpose

Encoding mouse Gnaq

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A recombinant mouse Gnaq (Gnaq Mouse)

Tags

N/A

Expression

Mammalian

Mutation

Asilent mutation was introduced into the sgRNA-ta…

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn GFP-Fxr1

Plasmid

#112732

Purpose

AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

Depositor

Martin Beaulieu

Article

Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

Insert

Fxr1p (Fxr1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

hSYN

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMVA2

Plasmid

#119951

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Expression

Bacterial

Mutation

A110G

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe DN-FOXO1-HA neo

Plasmid

#45814

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-U1AF37MF77M

Plasmid

#168267

Purpose

Expresses human dmU1A with the F37M/F77M double mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

U1A RRM1 crystallization module (SNRPA Human)

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F37M and F77M

Promoter

T7 promotor

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgLkb1/Cre

Plasmid

#66894

Purpose

Expresses an Lkb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgLkb1

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-CLEC4E

Plasmid

#172412

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member E (CLEC4E Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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