163,354 results

1. pY108 (lenti-AsCpf1)

   Plasmid

   #84739

   Purpose

   Lenti virus delivery of AsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRc/CMV HA TrkB

   Plasmid

   #39978

   Depositor

   Yves-Alain Barde

   Article

   Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

   Insert

   TrkB (Ntrk2 Rat)
   

   Use

   Tags

   BM40 signal sequence and HA

   Expression

   Mammalian

   Mutation

   endogenous signal sequence was replaced with BM40…

   Promoter

   CMV

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. p189

   Plasmid

   #65207

   Purpose

   Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

   Depositor

   LuZhe Sun

   Article

   Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. mCherry-ITSN1

   Plasmid

   #129614

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   ITSN1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-Flag-Timeless

   Plasmid

   #22887

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

   Insert

   Timeless (TIMELESS Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted normal start codon, replaced with start c…

   Promoter

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1E2

   Plasmid

   #20063

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E2 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeats of he…

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-nCas9n

   Plasmid

   #46757

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

   Plasmid

   #173030

   Purpose

   To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   SARS-CoV-2 Nucleocapsid phosphoprotein
   

   Use

   Lentiviral

   Tags

   3xHA and IRES-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   Gag (truncated)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVHA hEZH2

   Plasmid

   #24230

   Purpose

   Mammalian expression of human EZH2 with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM5Z(+)-EGFP

    Plasmid

    #65206

    Purpose

    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Restriction enzyme recognition sites added either…

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSIN-TRE-H2BeGFP-rtTA2

    Plasmid

    #165494

    Purpose

    Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

    Depositor

    Hector G. Palmer

    Article

    Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    -

    Promoter

    TetO-CMV

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163059

    Purpose

    mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDawn

    Plasmid

    #43796

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191182

    Purpose

    Expression of EACTB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:ACTB.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLminP_Luc2P_RE39

    Plasmid

    #90379

    Purpose

    PPARg - PPARg homodimer gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    RELB

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAK_DR30_EF1a_CasRx_Puro

    Plasmid

    #134842

    Purpose

    Lentiviral all in one expression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Tags

    T2A Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-miniGFP1-P2A-FusionRed

    Plasmid

    #188732

    Purpose

    Express miniGFP1 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORANGE GFP-Grin1 KI

    Plasmid

    #131485

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCV020-tetQ

    Plasmid

    #232826

    Purpose

    Random transposon mutagenesis vector for Bacteroidales species - tetracycline

    Depositor

    Athanasios Typas

    Insert

    tetQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge