19,834 results

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pRK7

Plasmid

#10883

Depositor

John Blenis

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET17_K560_GFP_His

Plasmid

#15219

Depositor

Ron Vale

Article

Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

Insert

Human neuronal kinesin heavy chain (KIF5B Human)

Tags

GFP and His

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pT2/BH

Plasmid

#26556

Depositor

Perry Hackett

Insert

pT2/BH

Expression

Bacterial

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-HA-UbcH13

Plasmid

#12461

Depositor

Dong-Er Zhang

Article

Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

Insert

Ubc13 (UBE2N Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pET-MBP-Geo_st

Plasmid

#87700

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

Depositor

Jennifer Doudna

Insert

GeoCas9

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EBFP2(1-10)

Plasmid

#165141

Purpose

Expresses EBFP2(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

EBFP2(1-10)

Expression

Mammalian

Available Since

April 8, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-C 16E6

Plasmid

#37876

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
pmCherry-C1_SEPT9 i1

Plasmid

#71622

Purpose

mammalian expression of human SEPT9 with an mCherry fusion

Depositor

Elias Spiliotis

Article

Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

Insert

Septin 9 (SEPTIN9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP2K1

Plasmid

#23406

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA4-GFP-Vinculin

Plasmid

#107153

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

(His)10-GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 IKKE K38A flag

Plasmid

#26202

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon K38A (IKBKE Human)

Tags

Flag

Expression

Mammalian

Mutation

Catalytic Lysine changed to Alanine (K38A)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223_NOTCH2_WT

Plasmid

#82292

Purpose

Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH2 (NOTCH2 Human)

Use

Gateway entry vector

Mutation

C946Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-H-Ras_wt

Plasmid

#39503

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Dre

Plasmid

#55633

Purpose

Expresses Dre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Inserts

mCherry
Dre

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human PP2A Aα full length

Plasmid

#132634

Purpose

expresses human PP2A Aα full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A Aα (PPP2R1A Human)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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N1-p18-EGFP

Plasmid

#42334

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
YIp204-PADH1-atTIR1-9myc

Plasmid

#99532

Purpose

S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

TIR1 (TIR1 Mustard Weed)

Tags

9xMyc

Expression

Yeast

Promoter

ADH1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

Plasmid

#141372

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

Plasmid

#160540

Purpose

Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Inserts

SARS-CoV-2 NSP7 (ORF1ab Synthetic)
SARS-CoV-2 NSP8 (ORF1ab Synthetic)
SARS-CoV-2 NSP12 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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