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PKN1 gRNA (BRDN0001162558)

Plasmid

#76278

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

Plasmid

#163545

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001144730)

Plasmid

#77980

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

Plasmid

#89607

Purpose

Expression of LIN28A in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

LIN28A (LIN28A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001487049)

Plasmid

#77981

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/ER-αT2ib

Plasmid

#110769

Purpose

Expresses ER intrabody against mouse and human TLR2

Depositor

Thomas Böldicke

Article

Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

Insert

intrabody against mouse and human TLR2

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-ePhi29 (LM2802)

Plasmid

#208967

Purpose

Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001162440)

Plasmid

#76140

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Slp76-SH2

Plasmid

#46503

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slp76 (LCP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-533

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

Plasmid

#162098

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to HA-FLAG-AU1

Use

Lentiviral

Tags

HA-FLAG-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

Plasmid

#162099

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC

Use

Lentiviral

Tags

VSVg-StrepTagII-ProtC

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU

Plasmid

#179415

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7.Chromo/TAF3.PHD-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PGK1 gRNA (BRDN0001146969)

Plasmid

#77982

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PFKFB3 gRNA (BRDN0001147151)

Plasmid

#76461

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB3 (PFKFB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PFKFB3 gRNA (BRDN0001147212)

Plasmid

#76463

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB3 (PFKFB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLV-shEsr2

Plasmid

#120722

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

Depositor

Amy Lasek

Article

Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

Insert

Esr2 (Esr2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.2-PGK-dTomato

Plasmid

#89605

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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