We narrowed to 88,800 results for: Mal

1. pCMV-RVR-A3A-N57G

   Plasmid

   #193657

   Purpose

   Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-N57G, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK_neo_3xflag_CHEK2

   Plasmid

   #136536

   Purpose

   Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   CHEK2 (CHEK2 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   3x FLAG

   Expression

   Mammalian

   Available Since

   June 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pStreptagIIx2 Alfatag 3C InsR EABR

   Plasmid

   #234996

   Purpose

   For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   InsR-EABR (INSR Human)
   

   Tags

   2xStreptag-II, Alfatag, HRV 3C site

   Expression

   Mammalian

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast FLVCR1b-3X FLAG

   Plasmid

   #218536

   Purpose

   human FLVCR1b with C-terminal 3X_FLAG cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Use

   Retroviral

   Tags

   FLAG (3X)

   Expression

   Mammalian

   Mutation

   Alternative isoform

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

   Plasmid

   #218534

   Purpose

   human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Use

   Retroviral

   Tags

   FLAG (3X)

   Expression

   Mammalian

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-SEMA3A-COMP5AP-AviTag-9xHis

   Plasmid

   #157536

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3A (SEMA3A Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

   Plasmid

   #218344

   Purpose

   Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-UCOE-SFFV-dCas9-P2A-EGFP

   Plasmid

   #188898

   Purpose

   Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and P2A-GFP

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188774

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_Xph20-mEos3.2-CCR5TC

    Plasmid

    #135532

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac3-EcLeu TAG-EGFP**

    Plasmid

    #228785

    Purpose

    EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcLeuRS
    EcLtR
    EGFP**
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac3-EcTrp-TGA-EGFP**

    Plasmid

    #228786

    Purpose

    EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    EGFP**
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-FKBP (YF)

    Plasmid

    #20175

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP (YF): (FKBP1A Human)
    

    Expression

    Mammalian

    Mutation

    FKBP was taken out from pC4M-F2E with a flanking …

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_Xph20-mNeonGreen-CCR5TC

    Plasmid

    #135534

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-shCELF4-4405

    Plasmid

    #115466

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shCELF4-4405 (CELF4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-S1-GCaMP6f

    Plasmid

    #160722

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG_Xph15-mNeonGreen-CCR5TC

    Plasmid

    #135533

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa-KIF5C 559-tdTomato-FKBP

    Plasmid

    #64211

    Purpose

    KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Kif5c (Kif5c Rat)
    

    Tags

    FKBP and tdTomato

    Expression

    Mammalian

    Mutation

    motor domain aa1-559

    Promoter

    chicken Beta Actin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP2443

    Plasmid

    #72252

    Purpose

    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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