We narrowed to 14,762 results for: RING

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J23114-mRFP1-331Bb

Plasmid

#78279

Purpose

mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23114-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-As

Plasmid

#138094

Purpose

Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH115gp160-QES.c14-754*

Plasmid

#123282

Purpose

Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (CH115) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(m40)-luc

Plasmid

#12425

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

PU.1 binding site mutation (created a KpnI site i…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pEMS1983

Plasmid

#49114

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pmiRGLO-BAX-201

Plasmid

#117332

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR BAX-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC101

Plasmid

#62335

Purpose

sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAG470 LifeAct-iFAST

Plasmid

#130821

Purpose

Expresses LifeAct-iFAST in mammalian cells (actin labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

LifeAct-iFAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP + uncleavable His10

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC73

Plasmid

#62341

Purpose

sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/L28F/G51PEKN

Plasmid

#118585

Purpose

Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, L28 mutated to F28, G51 mutat…

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

Plasmid

#101233

Purpose

Bacterial expression plasmid for SpCas9 Cluster 2 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant G582A/V583A/E584A/D585A/N588A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

G582A, V583A, E584A, D585A and N588A

Promoter

T7

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAG498 MAP4-iFAST

Plasmid

#130820

Purpose

Expresses MAP4-iFAST in mammalian cells (microtubule labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

MAP4-iFAST

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2177

Plasmid

#111892

Purpose

High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

Unspecified

Promoter

Ple344 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2175

Plasmid

#111890

Purpose

High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

Unspecified

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2176

Plasmid

#111891

Purpose

High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

Unspecified

Promoter

Ple343 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSC2

Plasmid

#91180

Purpose

T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting NCR genes

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CAG-Haunt-EF1a-DsRed

Plasmid

#92162

Purpose

Haunt in trans overexpression plasmid

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt (Halr1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP*

Plasmid

#41665

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM S297A

Plasmid

#169983

Purpose

Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM, S297A

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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