We narrowed to 66,749 results for: nin

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pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Tags

MGHHHHHHGGA

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Aqp4-eGFP

Plasmid

#176747

Purpose

Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Publication

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-BioID2-BioID2-HA

Plasmid

#176855

Purpose

Expresses the promiscuous small biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Publication

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CPSF1

Plasmid

#155475

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF1 (CPSF1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Electra2-P2A-EGFP

Plasmid

#184936

Purpose

Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-mCherry

Plasmid

#174443

Purpose

in vitro transcription template for dCas9-mCherry

Depositor

Michael Lampson

Publication

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

mCherry

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR_PGK_antiCD19_H1_Gal4VP64

Plasmid

#169918

Purpose

Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

Depositor

Zhili Rong

Publication

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch1-GL4VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSYC-437

Plasmid

#163829

Purpose

Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

Depositor

Seok-Yong Choi

Publication

Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

Inserts

Foxj1a Promoter
GCaMP6s

Use

Multisite gateway destination vector

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221_SLC52A3

Plasmid

#132181

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A3 (C20orf54 Human)

Expression

Mammalian

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13b-GGS-Sun

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-dDIO-vCre

Plasmid

#118029

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-dDIO-vCre

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-135

Plasmid

#118521

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-Wnt3a

Plasmid

#114287

Purpose

TET-inducible expression of Wnt3a

Depositor

Patrick Salmon

Publication

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Publication

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAB107a

Plasmid

#79218

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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e-ciCas9_pcDNA5

Plasmid

#100553

Purpose

Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Publication

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

e-ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Publication

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p006kanGFP

Plasmid

#58534

Purpose

GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

Depositor

Jaroslaw Bryk

Publication

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

T5 between two lac operators

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCasper4-tubulin-IVS-Syn21-QSco-p10

Plasmid

#46132

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QSco

Expression

Insect

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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