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Showing: 2121 - 2160 of 2761 results

  2. pAW41

    Plasmid
    #110228
    Purpose
    integration of loxP site a distance upstream of gene start site
    Depositor
    Tony Carr
    Article
    Watson et al Gene. 2008 Jan 15;407(1-2):63-74
    Insert
    ura4+ (ura4 Fission Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    See depositor comments below.
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    110228-plasmid-map-sequence-id-219790

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  6. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

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  8. pSCW02

    Plasmid
    #72301
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72301-plasmid-map-sequence-id-255198

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  9. pJH136

    Plasmid
    #47554
    Purpose
    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10
    Insert
    URA3
    Use
    Pcr template
    Tags
    Expression
    Mutation
    Promoter
    Native URA3 promoter, -243 to -1.
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    47554-plasmid-map-sequence-id-67878

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  11. pRS263

    Plasmid
    #122379
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Insert
    HSP90
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122379-plasmid-map-sequence-id-241246

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  12. pRS267

    Plasmid
    #122380
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Insert
    HSP90
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122380-plasmid-map-sequence-id-243382

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  15. pGBW-m4137386

    Plasmid
    #149540
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pmIL-6 -231

    Plasmid
    #61287
    Purpose
    drives luciferase via mouse IL-6 promoter, truncated (-231)
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    Truncated IL-6 promoter
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of 1046 nucleotides from the 5' end…
    Promoter
    IL-6 promoter (truncated)
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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  20. DLC1A

    Plasmid
    #25333
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DLC1:S1074-L1283 (DLC1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S1074-L1283
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pGBW-m4137387

    Plasmid
    #149544
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt;19-aa Ctrunc
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

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  26. pGGF002

    Plasmid
    #48843
    Purpose
    Provides a BASTA resistance cassette as GreenGate module.
    Depositor
    Jan Lohmann
    Article
    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:
    Insert
    p35S::BastaR::t35S
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    internal BsaI recognition site in 35S promoter re…
    Promoter
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    48843-plasmid-map-sequence-id-71254

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  28. pMT440

    Plasmid
    #8609
    Depositor
    Robert Hartley
    Article
    Yazynin et al Gene 1996 Feb 22;169(1):131-2.
    Insert
    barnase, barstar
    Use
    Tags
    as pMT416
    Expression
    Bacterial
    Mutation
    V36 codon of barnase of pMT416 replaced by polyli…
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  30. HOXC6 (human) HIS-tag pET

    Plasmid
    #8565
    Depositor
    Corey Largman
    Insert
    HOXC6 (HOXC6 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    This protein starts at an internal Met initially …
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  32. pDL12c

    Plasmid
    #17835
    Depositor
    Jay Keasling
    Article
    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72
    Insert
    gpdA promoter, lacZ, argB_1626,
    Use
    Synthetic Biology; Aspergillus nidulans expression
    Tags
    Expression
    Mutation
    The argB_1626 mutation consists of two sequential…
    Promoter
    Available since
    May 14, 2008
    Availability
    Academic Institutions and Nonprofits only

  33. pSU19mCherry

    Plasmid
    #225181
    Purpose
    Medium copy cloning vector with mCherry integrated in the multiple cloning site. There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    225181-plasmid-map-sequence-id-452505

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  34. pHBJT031

    Plasmid
    #225177
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    225177-plasmid-map-sequence-id-446510

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  36. pHBJT034

    Plasmid
    #225180
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225180-plasmid-map-sequence-id-444744

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  37. pHBJT032

    Plasmid
    #225178
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225178-plasmid-map-sequence-id-444695

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  38. pHBJT033

    Plasmid
    #225179
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225179-plasmid-map-sequence-id-444689

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  39. pHBJT030

    Plasmid
    #225176
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225176-plasmid-map-sequence-id-444686

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  40. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

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Showing: 2121 - 2160 of 2761 results