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pcDNA3_ATP10B-I1038T

Plasmid

#203700

Purpose

expresses ATP10B with the pathogenic mutation I1038T

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

I1038T

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-R153X

Plasmid

#203701

Purpose

expresses ATP10B with the pathogenic mutation R153X

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

R153X

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI_Hu_ECT2(T327D)

Plasmid

#136332

Purpose

Lentiviral expression of human ECT2_T327D

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T327D

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) PHLDB1

Plasmid

#107503

Purpose

Expresses PHLDB1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

PHLDB1 (PHLDB1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Nucleotide changes 1236 T>C, 1365 C>T, 1371…

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBFC0996

Plasmid

#186695

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
crRNA
Tn7 transposon
2xLguI Cargo Stuffer
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) SDC3

Plasmid

#107501

Purpose

Expresses SDC3, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

SDC3 (codon optimized) (SDC3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pM2AH + Slo1

Plasmid

#16195

Depositor

Larry Salkoff

Article

McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

Insert

calcium-activated potassium channel (KCNMA1, Kcnma1 Mouse, Human)

Expression

Mammalian

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-V5_Hu_p115-RhoGEF(WT)

Plasmid

#136335

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal V5

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSBtet-2xMARS-nGFP-puro

Plasmid

#205239

Purpose

DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Transposon

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

tight TRE promoter

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) MXRA7

Plasmid

#107500

Purpose

Expresses MXRA7, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

MXRA7 (codon optimized) (MXRA7 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-MARS-p85(iSH2)

Plasmid

#205236

Purpose

Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Inserts

PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
p85(iSH2) (Pik3r1 Mouse)

Tags

Nuclear Export Sequence and mCherry

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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CD59-CD4d3+4-bio

Plasmid

#73117

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD59 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD59 glycoprotein (CD59, MIC11 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B WT

Plasmid

#171770

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B WT

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Mutation

D433N

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBScMXT + Slo1

Plasmid

#16194

Depositor

Larry Salkoff

Article

McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

Insert

calcium-activated potassium channel (KCNMA1, Kcnma1 Mouse, Human)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) VOPP1

Plasmid

#107507

Purpose

expressing VOPP1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

VOPP1 (VOPP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B D433N

Plasmid

#171823

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge