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pFA6a-His3MX6

Plasmid

#41596

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSC

Plasmid

#46896

Purpose

expression of scrambled shRNA

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

scrambled

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS8c_ddCpf1_final

Plasmid

#212040

Purpose

Bacterial expression of the CRISPRi system for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

ddCpf1

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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Arp3-TagRFP

Plasmid

#195091

Purpose

Gene Overexpression of Human Arp3

Depositor

Jukka Westermarck

Article

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

Arp3 (APOBEC3A Human)

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Article

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_GFP

Plasmid

#127108

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

GFP

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pT2SC

Plasmid

#59382

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRSET-QNA

Plasmid

#129305

Purpose

Negative control for ATP measurement

Depositor

Hiroyuki Noji

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Double UP mClover3 to mScarlet

Plasmid

#120262

Purpose

Natively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mClover3/mScarletP2A-mcs

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Talin1

Plasmid

#26724

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRD119 (PCNA GFP)

Plasmid

#14822

Depositor

Robert Duronio

Article

Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Insert

PCNA-GFP (PCNA Fly)

Tags

GFP

Expression

Bacterial

Available Since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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