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pDEST40-2XFL-Src-K298M-Y419F-Y530F

Plasmid

#140321

Purpose

Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Publication

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v6-Fc(IgG1)

Plasmid

#145169

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 6

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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G31, Cdc20-AID tagging

Plasmid

#130269

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Publication

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Tags

AID(71-114)

Expression

Yeast

Promoter

Native promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hPMLIII

Plasmid

#103825

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Publication

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hPMLIII (CRY2, PML Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Rat SERCA2b (pMT2)

Plasmid

#75184

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, Gary Shull

Publication

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Expression

Mammalian

Promoter

adenovirus major late promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
hu-TLR4 299 snp-flag pDEST40

Plasmid

#42647

Purpose

destination vector for expression of human TLR4 299 SNP in mammalian cells

Depositor

Scott Friedman

Publication

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 299 SNP (TLR4 Human)

Tags

Flag

Expression

Mammalian

Mutation

D299G

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-GFP-IMP2-2_antisense-control

Plasmid

#42174

Depositor

Alexandra Kiemer

Publication

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pmyc-GFP-TNRC6A-NLS/NES-mut

Plasmid

#42002

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

Promoter

CMV

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST47-CypB-Iso C-term GFP

Plasmid

#36130

Depositor

Patrick Van Oostveldt

Publication

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 47-204 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST47-CypB-Golgi C-term GFP

Plasmid

#36128

Depositor

Patrick Van Oostveldt

Publication

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1-211 of cyclophilin B

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-BcrGAP R1090A

Plasmid

#38176

Depositor

Nora Heisterkamp

Publication

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

aa 1004-1271 with R1090A point mutation

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag IHPK2

Plasmid

#20538

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IHPK2 (IP6K2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ADCK4

Plasmid

#20544

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADCK4 (ADCK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag NEK3

Plasmid

#20547

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NEK3 (NEK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CKMT2

Plasmid

#20554

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKMT2 (CKMT2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PCTK3

Plasmid

#20562

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PCTK3 (CDK18 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MVK

Plasmid

#20616

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MVK (MVK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag STK32A

Plasmid

#20638

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

STK32A (STK32A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only