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pTH728-CEN-RLuc/maxCFLuc

Plasmid

#38212

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

Plasmid

#113953

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, I132S, S212N, and …

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTH726-CEN-RLuc/minCFLuc

Plasmid

#38210

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114104

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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[UBC][EIF4e][GG cloning]

Plasmid

#97189

Purpose

EIF4e plasmid with Pum cloning site

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

EIF4E variant 1 (EIF4E Human)

Use

Synthetic Biology

Tags

empty Pum cloning site

Expression

Mammalian

Promoter

UBC (ubiquitin C)

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTER GAMMA-TUBULIN shRNA Human

Plasmid

#87955

Purpose

reduced the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

sh gamma-tubulin (TUBG1 Human)

Tags

No-tag

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene.…

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
PB-PE VQR

Plasmid

#162797

Purpose

All-in-one prime editor piggyBac transposon, VQR variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Promoter

EF-1-alpha

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-saBE3.9max_KKH C-terminal

Plasmid

#137181

Purpose

Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-saBE3.9max_KKH_C-terminal

Expression

Mammalian

Mutation

E782K;N968K;R1015H conferring recognition of NNNR…

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3 delta Exon2

Plasmid

#100782

Purpose

Mammalian expression of BNIP3 delta Exon2 splice variant

Depositor

Joseph Gordon

Article

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 delta Exon2 (BNIP3 Human)

Tags

HA

Expression

Mammalian

Mutation

Exon2 deletion

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-CCR5(R5gp120-Hi)

Plasmid

#98954

Purpose

Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTH744-CEN-RLuc/slowmaxCFLuc

Plasmid

#38224

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

G168D,Y169D

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTH742-CEN-RLuc/slowminCFLuc

Plasmid

#38222

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge