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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 10841 - 10860 of 21642 results

  1. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

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  3. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid
    #208574
    Purpose
    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/CMV and minP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208574-plasmid-map-sequence-id-413226

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  7. pBbE1k-3

    Plasmid
    #71722
    Purpose
    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human
    Depositor
    Taek Soon Lee
    Article
    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1
    Inserts
    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    71722-plasmid-map-sequence-id-129984

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  8. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid
    #117720
    Purpose
    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    mAID-nanobody
    IBB-mCherry
    Use
    Tags
    importin-beta bining domain (IBB) and triple HA
    Expression
    Mammalian
    Mutation
    codon optimized mAID for murine expression
    Promoter
    CMV and None, IRES-driven
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117720-plasmid-map-sequence-id-227032

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  10. pET24a-VHH-APEX2

    Plasmid
    #109420
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109420-plasmid-map-sequence-id-211586

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  11. pdRBFC-B2-YN

    Plasmid
    #74721
    Purpose
    Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    HA and YFP N-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74721-plasmid-map-sequence-id-329048

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  14. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Promoter
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  15. pdRBFC-B2-YC

    Plasmid
    #74722
    Purpose
    Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    FLAG and YFP C-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74722-plasmid-map-sequence-id-329052

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  17. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100838
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1 and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100838-plasmid-map-sequence-id-198300

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  20. ZJOM113

    Plasmid
    #133666
    Purpose
    Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133666-plasmid-map-sequence-id-268963

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Showing: 10841 - 10860 of 21642 results