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piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

Plasmid

#209084

Purpose

Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

hSyn

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET30-2-GPD1

Plasmid

#83911

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pExp-His-zBasic-RBD

Plasmid

#194999

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli

Depositor

Marko Hyvönen

Article

Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

Insert

RBD (S SARS-CoV-2)

Tags

His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 PDZD8-WT

Plasmid

#227849

Purpose

To express in E. coli PDZD8-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Tags

GST

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC FLNA

Plasmid

#227876

Purpose

Used for transfection of cells for expression of C-MYC FLNA

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

FLNA (Flna Mouse)

Tags

MYC

Expression

Mammalian

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET30-2-GPD1L

Plasmid

#83912

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH

Plasmid

#83907

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223-K-RAS V12

Plasmid

#31200

Purpose

Gateway donor vector containing KRAS G12V.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway donor vector

Mutation

G12V constitutively active

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ILK

Plasmid

#20505

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ILK (ILK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP3K7

Plasmid

#20520

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K7 (MAP3K7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Q356H

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-sgPHGDH-G5

Plasmid

#83913

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag ITK

Plasmid

#20507

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ITK (ITK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK13

Plasmid

#20523

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK13 (MAPK13 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
elavl3:SomaGCaMP7f

Plasmid

#158760

Purpose

Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

Synthetic Biology

Promoter

HuC promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP2K5

Plasmid

#20514

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K5 (MAP2K5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK12

Plasmid

#20592

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK12 (MAPK12 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HK1

Plasmid

#20612

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK1 (HK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLEX-SomaGCaMP7

Plasmid

#182941

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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