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60,655 results

Showing: 10861 - 10880 of 60655 results

  5. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  6. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  7. FFA2-DuET

    Plasmid
    #213238
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FFA2 (FFAR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213238-plasmid-map-sequence-id-430796

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  8. pLV-Kif5b-T92N-Myc

    Plasmid
    #186615
    Purpose
    Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)
    Use
    Lentiviral
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    T92N
    Promoter
    CMV
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186615-plasmid-map-sequence-id-371213

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  9. pET28a-XPO5-His6

    Plasmid
    #171562
    Purpose
    Encodes human XPO5 protein
    Depositor
    Yan Zeng
    Insert
    XPO5 (XPO5 Human)
    Expression
    Bacterial
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171562-plasmid-map-sequence-id-337920

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  16. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Available Since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  17. CYP51A1 (3I3K)

    Plasmid
    #25161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP51A1_cw_09 (CYP51A1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 54-502
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. His 3C MKK6 1-334

    Plasmid
    #87881
    Purpose
    E. coli expression of MKK6 aa 1-334
    Depositor
    Peter Mace
    Insert
    MKK6 (MAP2K6 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-334
    Promoter
    T7
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87881-plasmid-map-sequence-id-174845

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Showing: 10861 - 10880 of 60655 results