Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,989 results for: T7

Clear keywords
Showing: 10881 - 10900 of 10989 results

  3. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid
    #136688
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression w/ three mut…
    Promoter
    T7
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136688-plasmid-map-sequence-id-272126

    Enlarge

  6. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

    Enlarge

  10. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104495
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    104495-plasmid-map-sequence-id-200303

    Enlarge

  13. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162685-plasmid-map-sequence-id-320886

    Enlarge

  14. pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE

    Plasmid
    #105322
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV1
    Insert
    jGCaMP7c variant 1513
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    105322-plasmid-map-sequence-id-213536

    Enlarge

  16. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162681-plasmid-map-sequence-id-321444

    Enlarge

  17. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

    Enlarge

  19. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

    Enlarge

  20. PET.SUMO eIF4G1 1-200

    Plasmid
    #158791
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (codon optimized)
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available Since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158791-plasmid-map-sequence-id-309813

    Enlarge

Showing: 10881 - 10900 of 10989 results