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Plasmid#236076PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidMutationG12VPromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only
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H2B-Grx1-roGFP2
Plasmid#236550PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-Grx1-roGFP2 (H2BC21 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti Puro HA-Ubiquitin-K48
Plasmid#236290PurposeLentiviral vector expressing HA-tagged ubiquitin K48 mutantDepositorInsertUbiquitin K48 (UBB Human)
UseLentiviralTagsHAMutationSingle ubiquitin coding sequence containing K48 m…PromoterCMVAvailable sinceApril 10, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI5-CasRx-NES-HA (pKW387)
Plasmid#229048PurposeMammalian expression of CasRx-NES-HADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCasRx (RfxCas13d)
UseCRISPRTagsNES-HAExpressionMammalianAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPPromoterCMVAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGAT-VH-hIgG1-CH1-TS-HIS
Plasmid#218436Purposemammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domainDepositorInsertvGAT-Vh-hIgG1-CH1
UseAffinity Reagent/ AntibodyTagsBM40 and TwinStrep-6xHisExpressionMammalianPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR97-DuET
Plasmid#213294PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR97 (ADGRG3 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR6-DuET
Plasmid#213223PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR6 (CXCR6 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mSTING (V154M)
Plasmid#214159PurposeTransient expression of gain-of-function STING mutantDepositorAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-nuCPE
Plasmid#213748PurposeFor circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCP-LbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R in LbCas12aPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2h
Plasmid#208709PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
ExpressionMammalianPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1280 NLS-mCherry-FIREtag-NES
Plasmid#205172Purposeexpresses NLS-mCherry-FIREtag-NES in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-mCherry-FIREtag-NES
ExpressionMammalianAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB104
Plasmid#199314PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
ExpressionWormMutationmTagBFP2 from pJJR81, C. elegans codon optimized …Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD45
Plasmid#203753PurposeEncodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttrCD45 (PTPRC Human)
TagsmEos3.2ExpressionMammalianAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-C1-FKBP-CYB5tail
Plasmid#202686Purposerapamycin-induced recruitment of proteins to ERDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
ExpressionMammalianAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD
Plasmid#193823PurposeCMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert[4xCox8]-ChemoG-NAD
ExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-NAD
Plasmid#193824PurposeCMV overexpression of ChemoB-NAD in mammalian cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChemoB-NAD
ExpressionMammalianMutationEBFP2:N39Y, A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-H159Q-HA
Plasmid#198183PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) H159QDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACOD1 (ACOD1 Human)
TagsHAExpressionMammalianMutationThe histidine in IRG1 at position 159 mutates to …PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-L272Q-HA
Plasmid#198184PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) L272QDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACOD1 (ACOD1 Human)
TagsHAExpressionMammalianMutationThe leucine in IRG1 at position 272 mutates to gl…PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only