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EC71167

Plasmid

#154066

Purpose

Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct

Use

Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Zea mays Ubiquitin Promoter

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFSynW Substance P-pHluorin IRES mRuby3

Plasmid

#195699

Purpose

Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Tac1 (Tac1 Mouse)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RABIF

Plasmid

#130882

Purpose

Expresses human RABIF with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RABIF (RABIF Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-Squ:mCherry

Plasmid

#20163

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pTA231-p.Syn-Dendra2Hfx

Plasmid

#164660

Purpose

expression of H. volcanii codon-optimized Dendra2

Depositor

Anita Marchfelder

Article

Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

Insert

Dendra2Hfx

Use

Archaeal expression

Mutation

codon-optimization for Haloferax volcanii

Promoter

p.Syn

Available Since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-LC3BGlydelta5C

Plasmid

#190237

Purpose

Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1 ASA

Plasmid

#13479

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1ASA (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Arginine 53 to Alanine, Changed Arginine…

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pTCO2-mTDGi.0

Plasmid

#149429

Purpose

miniTdg gene

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

SNPs of the Tdg gene from the OLA/129 strain

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFIN-XOPS-tdTOM-MOPS-GFP

Plasmid

#44347

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

XOPS-tdTOM
MOPS-GFP

Use

Lentiviral

Promoter

Xenopus Opsin (XOPS) (pos 3131-4495) and mouse op…

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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139H2_LC

Plasmid

#206202

Purpose

For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

Depositor

Joost Snijder

Article

Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

Insert

anti-MUC1 antibody 139H2 light chain (Igkc Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-4x

Plasmid

#124684

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) G361C

Plasmid

#134694

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

G361C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
TRE-UBC-Crry-T2A-GFP

Plasmid

#205452

Purpose

lentiviral plasmid for expression of mouse Crry

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Crry (Cr1l Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBScMXT + mSlo1 MBr5/3

Plasmid

#16149

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse brain)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pBS-Squ5'3'

Plasmid

#20165

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
mKikGR-Tubulin-6

Plasmid

#57330

Purpose

Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Tubulin (TUBA1B Human)

Tags

mKikGR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-EGFP-C1-HsDCP2-F404AF407AF409A_V

Plasmid

#147813

Purpose

Mammalian Expression of HsDCP2-F404AF407AF409A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-F404AF407AF409A (DCP2 Human)

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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