159,428 results

1. pTEF:ATP

   Plasmid

   #92179

   Purpose

   HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
   

   Use

   Integration and expression into yeast.

   Tags

   Yeast codon optimized mseCFP (donor) and cp173-mV…

   Expression

   Yeast

   Mutation

   Promoter

   TEF1p

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7_zntR

   Plasmid

   #216324

   Purpose

   T7 promoter driving expression of zntR

   Depositor

   Mark Styczynski

   Article

   McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

   Insert

   Zinc-responsive activator
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

   Plasmid

   #194685

   Purpose

   CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RCASBP-AP (A) (CC#37)

   Plasmid

   #15160

   Depositor

   Connie Cepko

   Article

   Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

   Insert

   PLAP (ALPP Human)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTPRC-bio-His

   Plasmid

   #51813

   Purpose

   Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PTPRC (PTPRC Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-21a-PE2-His

   Plasmid

   #201973

   Purpose

   Express His-tagged PE2 in bacteria

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   PE2-HIS
   

   Use

   Tags

   His and c-myc NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. htrpC6-YFP

   Plasmid

   #21084

   Depositor

   Craig Montell

   Article

   Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

   Insert

   TrpC6 (TRPC6 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ARTX_ctrl

   Plasmid

   #214065

   Purpose

   Fluorescent reporter system, NMD negative control. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   TurboRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. hATMS1981A

    Plasmid

    #32300

    Depositor

    Michael Kastan

    Article

    Bakkenist et al Nature. 2003 Jan 30;421(6922):499-506.

    Insert

    ATM (ATM Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    S1981A

    Promoter

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IZ-ANXA6-mRuby3

    Plasmid

    #184892

    Purpose

    Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA6 (ANXA6 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R587K

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Vamp2-pHluorin

    Plasmid

    #190151

    Purpose

    Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-HA_WT RAD23A

    Plasmid

    #201458

    Purpose

    Expresses human RAD23A with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag p38 delta

    Plasmid

    #20785

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    pDCNA3 Flag p38 delta (Mapk13 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. loSIIN

    Plasmid

    #185663

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS162

    Plasmid

    #194485

    Purpose

    synGR Cassette

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    synGR Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-P301L Tau

    Plasmid

    #176267

    Purpose

    This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

    Depositor

    Tsuneya Ikezu

    Article

    You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

    Insert

    Flex-P301L tau (MAPT )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Clover

    Plasmid

    #40259

    Purpose

    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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