We narrowed to 17,223 results for: EMB

Showing: 10921 - 10940 of 17223 results

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pX330-EZR

Plasmid

#227293

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of EZR (EZR Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CEP192

Plasmid

#227288

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CEP192 (CEP192 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAG155

Plasmid

#226744

Purpose

CRISPRi guide RNA against TOMM20

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM20 (TOMM20 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG156

Plasmid

#226745

Purpose

CRISPRi guide RNA against TOMM20

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM20 (TOMM20 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG392

Plasmid

#226749

Purpose

CRISPRi guide RNA against ATP13A1

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

ATP13A1 (ATP13A1 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG369

Plasmid

#226748

Purpose

CRISPRi guide RNA against EMC2

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

EMC2 (EMC2 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG517

Plasmid

#226735

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-EF1a-pA (JDW 1222)

Plasmid

#224526

Purpose

A Gateway compatible 3' entry clone containing rat EF-1a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF-1a polyA

Use

Gateway cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p5E_hs_Glast (JDW 1182)

Plasmid

#224483

Purpose

Gateway compatible 5' entry clone containing the Human GLAST promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

GLAST promoter

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSG071

Plasmid

#214257

Purpose

Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSG075

Plasmid

#214258

Purpose

Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSG086

Plasmid

#214263

Purpose

PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M

Tags

6x His-tag

Expression

Bacterial

Mutation

substitution of 31 amino acids of FtsH2 (F124RRFA…

Promoter

T7 promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hs_Cldn5 (JDW 1120)

Plasmid

#224481

Purpose

Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Claudin-5 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME AmCyan (JDW 1151)

Plasmid

#224485

Purpose

Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

AmCyan

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5 mScarlet-I (JDW 1323)

Plasmid

#224487

Purpose

Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1367)

Plasmid

#224503

Purpose

Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC (no LexOp) (JDW 937)

Plasmid

#224504

Purpose

Gateway compatible middle entry clone containing a destabilized LexA transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myc-BirA (JDW 1210)

Plasmid

#224509

Purpose

Gateway compatible middle entry clone containing a Myc tagged BirA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BirA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS1 (JDW 1095)

Plasmid

#224510

Purpose

Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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