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pMSCV-IRES-DsRed/MPL

Plasmid

#214793

Purpose

Mammalian expression of human MPL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL (MPL Human)

Use

Retroviral

Tags

non

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet-KASH_NoMBS

Plasmid

#231014

Purpose

CVS N2c RVdG genome plasmid, utilized to generate negative control virus to benchmark the utility of MS2 tagging for capture of barcodes using snRNA-seq.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-CKS2-HA

Plasmid

#247439

Purpose

HA-tagged mouse CKS2 cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

CKS2 (Cks2 Mouse)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-CDKL5(isoform-2)

Plasmid

#245901

Purpose

Mammalian expression of CDKL5 isoform2 (1-1030) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-1 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-CDKL5(kinase)

Plasmid

#245902

Purpose

Mammalian expression of CDKL5 kinase domain (1-311) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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tetO-Mex3a_HyperTRIBE-adar_deaminase-V5-t2a-mCherry

Plasmid

#241315

Purpose

inducible Mex3a fused to mouse Adar Deaminase domain (HyperTRIBE) and V5 with t2a mCherry for expression in mammalian cells

Depositor

Stavros Lomvardas

Inserts

Mex3a (Mex3a Mouse)
Adar deaminase domain (Adar Mouse)

Tags

Adar_deaminase_domain and V5

Expression

Mammalian

Mutation

E957Q

Promoter

tetO and CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CDKL5(isoform1)-GFP

Plasmid

#245903

Purpose

Mammalian expression of CDKL5 isoform1 (1-960) with C-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHCFnc/V5

Plasmid

#247003

Purpose

Expresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoter

Depositor

Thomas Kristie

Article

Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

Insert

HCF-1 (HCFC1 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

Glu to Ala change at amino acid positions 1019,10…

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem

Plasmid

#241393

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-ETV2; rtTA-T2A-mTagBFP2 (ETV2 Human)

Tags

NLS

Expression

Mammalian

Promoter

TRE; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-sfGFP-TurboID-ER

Plasmid

#240232

Purpose

Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_spatzle-HA

Plasmid

#240227

Purpose

Gateway entry clone with spatzle tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

spatzle (spz Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrnp48_i1-ipUSEPR-TR657

Plasmid

#228925

Purpose

Knockdown of Snrnp48 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrnp48_i1 (Snrnp48 Mouse)

Use

Lentiviral

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD 11W D262V

Plasmid

#234618

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_11W D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

F202W, F210W, F216W, F222W, F228W, Y237W, Y244W, …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0176 CMVTO-mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra

Plasmid

#231202

Purpose

CageIL-10-mono (mouse renin cut-site)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Tada3_i3-ipUSEPR-TR657

Plasmid

#228915

Purpose

Knockdown of Tada3 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Tada3_i3 (Tada3 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Tada3_i5-ipUSEPR-TR657

Plasmid

#228916

Purpose

Knockdown of Tada3 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Tada3_i3 (Tada3 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Snrnp48_i4-ipUSEPR-TR657

Plasmid

#228926

Purpose

Knockdown of Snrnp48 in mammalian cell

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrnp48_i4 (Snrnp48 Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S724A

Plasmid

#224735

Purpose

For expression of HA-tagged GTSE1 protein with S724A mutation

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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