We narrowed to 22,456 results for: his

Showing: 10961 - 10980 of 22456 results

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUB6-VSV-GS

Plasmid

#190781

Purpose

Expresses VSV-GS in mammalian cells

Depositor

Jakob Reiser

Article

Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

Insert

VSV-GS

Tags

HA

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#190508

Purpose

Mammalian Expression of Bassoon scFV. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Bassoon (Rattus norvegicus) recombinant scFV (Bsn Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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SPHKAP scFv [L131/17]

Plasmid

#190511

Purpose

Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/13]

Plasmid

#190498

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Nav1.8 Na+ channel scFv [N134/12]

Plasmid

#190530

Purpose

Mammalian Expression of Nav1.8 Na+ channel scFV. Derived from hybridoma N134/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Nav1.8 Na+ channel (Rattus norvegicus) recombinant scFV (Scn10a Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin (extracellular) scFv [A12/18]

Plasmid

#190485

Purpose

Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-cyScarlet

Plasmid

#191260

Purpose

Bacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarlet

Depositor

Robert Campbell

Insert

cyScarlet

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Article

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pH6-spTorA-mCherry

Plasmid

#169040

Purpose

mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-mCherry

Tags

6xHis and spTorA (signal peptide of E. coli TorA)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-tdTEVDB_C-15A/G4C

Plasmid

#177205

Purpose

Permuted td intron with evolved initiation sequence for the expression of circular mRNA

Depositor

Stephen Fried

Article

Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

Insert

Permuted GFP with evolved initiation sequence for circular mRNA

Tags

6x His

Expression

Bacterial

Mutation

Permuted sfGFP after residue 52 & Changed Cyt…

Promoter

araBAD

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cut-P(11)4

Plasmid

#185785

Purpose

Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

Cut-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSN840

Plasmid

#184832

Purpose

Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Inserts

KIF5A (∆exon27) (KIF5A Human)
KLC1 (KLC1 Human)

Use

Cre/Lox

Tags

His-FLAG and mScarlet-2xStrepII

Expression

Insect

Mutation

∆exon27 form of human KIF5A

Promoter

p10 and polyhedrin

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-11_enzymatic-domain

Plasmid

#183382

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-11 (AA100-373) (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

Plasmid

#181956

Purpose

Genetically encoded FRET-based cAMP indicator fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)

Tags

AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-mClover3-CD4-bla

Plasmid

#179441

Purpose

Donor vector to knock in mClover3 C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mClover3

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-Halo-CD4-bla

Plasmid

#179445

Purpose

Donor vector to knock in Halotag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Mortalin/GRP75 scFv [N52A/42]

Plasmid

#182091

Purpose

Mammalian Expression of Mortalin/GRP75 scFv. Derived from hybridoma N52A/42.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Mortalin/GRP75 (Mus musculus) (Hspa9 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Cav1.2 Ca2+ channel scFv [N263/31]

Plasmid

#182083

Purpose

Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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