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Addgene

We narrowed to 8,806 results for: ef1;

Showing: 2701 - 2750 of 8806 results
  1. pXP420 v2 (repaired Amp promoter)

    Plasmid
    #86920
    Purpose
    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. R777-E226 Hs.RAPGEF2-nostop

    Plasmid
    #70510
    Purpose
    Gateway ORF clone of human RAPGEF2 [NM_014247.2] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    RAPGEF2 (RAPGEF2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. Aiolos in Artichoke

    Plasmid
    #74452
    Purpose
    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Insert
    Aiolos (IKZF3) (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid
    #110568
    Purpose
    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
    Insert
    3xFLAG/HA-tagged anti-GFP VHH
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. Aiolos AA130-189 in Artichoke

    Plasmid
    #74451
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pJA291

    Plasmid
    #74487
    Purpose
    eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP
    Depositor
    Insert
    puroR-mCherry-T2A-eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pC0037

    Plasmid
    #181934
    Purpose
    Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)
    Depositor
    Inserts
    Gaussia luciferase
    Cypridina luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1alpha
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pLenti-EGFP-hChibby1

    Plasmid
    #89770
    Purpose
    Labels mother centrioles/basal bodies
    Depositor
    Insert
    Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1alpha
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pJAID8Z

    Plasmid
    #165053
    Purpose
    Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.
    Depositor
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pILGFP105

    Plasmid
    #165060
    Purpose
    Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1
    Depositor
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pJAID9L104

    Plasmid
    #165054
    Purpose
    Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.
    Depositor
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2701 - 2750 of 8806 results