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Plasmid#75176PurposeMembrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertmTagBFP-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB17
Plasmid#52527Purposeserves as template for PCR-mediated fusion of U6-promoter with sgRNADepositorInsertDrosophila U6C promoter
UseCRISPR; Blunt cloning kitTagsT7-promoterExpressionInsectMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-mCherry-NS-MCS
Plasmid#75179PurposemCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertmCherry-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD200RCd4d3+4-bio
Plasmid#32406PurposeA positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kitDepositorInsertCD200 Receptor extracellular domain (Cd200r1 Rat)
UseTagsCd4d3+4 and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-mKOFP2-CAAX
Plasmid#75155PurposeMembrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.DepositorInsertmKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD200RCd4d3+4-blac
Plasmid#32407PurposeA positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kitDepositorInsertCD200 Receptor extracellular domain (Cd200r1 Rat)
UseTagsCd4d3+4 and beta-lactamaseExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pmyo3::tag:sl1(G16C)
Plasmid#79003PurposeExpression of exogenous SL1 in C. elegans muscleInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG16CPromotermyo-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(3'')-Ia
Plasmid#112879PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertaph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD200-Cd4d3+4-COMP-blac-3xFLAG-6his
Plasmid#71471PurposeA positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kitDepositorInsertCD200 (Cd200 Rat)
UseTags6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHW394 (15xUAS::GFP::let-858 3'UTR)
Plasmid#85584PurposecGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegansDepositorInsert15xUAS-delta pes-10-GFP
UseTagsExpressionWormMutationGFP(S65C) with three introns for expression in C.…Promoter15xUAS-delta pes-10-GFPAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-loxP-mTagBFP2-stop-pA-loxP
Plasmid#75158PurposeFloxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.DepositorInsertloxP-mTagBFP2-stop-pA-loxP
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD
Plasmid#37275PurposeTALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)DepositorTypeEmpty backboneUseTagsFlag, Fok I DD, Tal-C, and Tal-NExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS
Plasmid#154229PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-CreLite
Plasmid#131782PurposeMiddle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.DepositorInsertPhyBCreC-P2A-PIF6CreN
UseCre/Lox; Gateway cloningTagsExpressionMutationPromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pu2-3::tag::sl1(G16C)
Plasmid#79002PurposeExpression of exogenous SL1 in multiple tissues of C. elegansInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG16CPromoterF08G2.17Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS
Plasmid#154223PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 11xQUAS+CP_pcbCDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS
Plasmid#154238PurposeEncodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbCDepositorInsertpgndA:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterpGndA (An11g02040 A. niger)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B-Pho2-1/2-2-tRNA
Plasmid#161761PurposeMODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)DepositorInsertgRNAs
UseTagsExpressionBacterialMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only