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pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

Plasmid

#155125

Purpose

Tol2 vector containing Cre-inducible QFGal4-switch

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4-switch

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mutation

Promoter

Ubi

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GRAB_eCB2.0

Plasmid

#164608

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Tags

Expression

Mutation

S383T

Promoter

CaMKII

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLacO-pEF1α-RFP

Plasmid

#179507

Purpose

Expresses monomeric RFP in mammalian cells under a EF1α promoter

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

monomeric RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP682-Smad2

Plasmid

#118943

Purpose

Mammalian expression of human Smad2 protein

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

iRFP682-Smad2 (SMAD2 Human)

Use

Tags

iRFP682

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

Plasmid

#138419

Purpose

CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Expression

Mutation

Promoter

EF1a

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_pSyn_Mito-LOV-Turbo-V5

Plasmid

#199669

Purpose

expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

COX4I1 mito targeting sequence and V5

Expression

Mutation

Promoter

Synapsin

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_PP7-mVenus

Plasmid

#111268

Purpose

Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

PP7-mVenus

Use

Synthetic Biology

Tags

HA, SV40 NLS, and mVenus

Expression

Mammalian

Mutation

PP7: C68S, S69G [delta]70-75 (non-aggregating)

Promoter

hUBC

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAL204

Plasmid

#22282

Purpose

Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Use

Tags

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Promoter

Available since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
FKBP-alpha(740-977)-GFP

Plasmid

#100731

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2A1 (Ap2a1 Mouse)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pscAAV-Tyr

Plasmid

#135616

Purpose

Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI RhoA

Plasmid

#189669

Purpose

Expression of Antenna-RhoA

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

RhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Use

Tags

2xSV40-NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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LightR-iCre-miRFP670

Plasmid

#162158

Purpose

Expresses LightR-iCre-miRFP670 in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

LightR-iCre-miRFP670

Use

Tags

NLS and miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Rac1

Plasmid

#83359

Purpose

Expression of Antenna-Rac1

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Rac1 (RAC1 Human)

Use

Tags

FRET Antenna (mCerulean-cpVenus) and His

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mGrn-myc

Plasmid

#178944

Purpose

Construct for expressing murine progranulin (Grn) in AAV

Depositor

Erik Roberson

Article

Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

Insert

Grn (Grn Mouse)

Use

AAV

Tags

Myc

Expression

Mutation

Promoter

CBA

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pXY349

Plasmid

#200262

Purpose

Expressing FtsW-TagRFP-T in bacterial cells

Depositor

Jie Xiao

Article

Yang et al Nat Microbiol. 2021 May;6(5):584-593. do

Insert

FtsW-tagRFP (ftsW )

Use

Tags

tagRFP

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Use

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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