We narrowed to 4,313 results for: Pet2

1. VV001: WT Arch-3 in pET28b

   Plasmid

   #58487

   Purpose

   Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

   Depositor

   Adam Cohen

   Article

   Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Archaerhodopsin-3 (with C-terminal His tag)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-10

   Plasmid

   #172743

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-10
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_human-pro-IL-18_DeltaN24

   Plasmid

   #214327

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Deletion of first 24 amino acids

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_Caspase-5-enzymatic-domain

   Plasmid

   #214306

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP5 (CASP5 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   aa133-434

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-42

   Plasmid

   #172758

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-42
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-trAtCCD8(pYL735)

   Plasmid

   #178286

   Purpose

   Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-GFP-AURKA

   Plasmid

   #99875

   Purpose

   Expression of GFP-AURKA for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-PTBP1-RRM34

   Plasmid

   #89156

   Purpose

   Recombinant expression of PTBP1-RRM34 in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   contains RNA Recognition Motif 3+4

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-TEV-U1AF37MF77M

   Plasmid

   #168267

   Purpose

   Expresses human dmU1A with the F37M/F77M double mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   U1A RRM1 crystallization module (SNRPA Human)
   

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F37M and F77M

   Promoter

   T7 promotor

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24a-VHH-2xTS

    Plasmid

    #109419

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-6

    Plasmid

    #172741

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-6
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-a(+)-6xHis-LSSmCherry1(-13)

    Plasmid

    #219415

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    LSSmCherry1(-13)
    

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    K137E, K199E

    Promoter

    T7

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET23-CBD-(-PP)-EGFP

    Plasmid

    #12597

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    Cdc42 Biosensor (WAS Human)
    

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    F271C

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET26-Ufd1-His

    Plasmid

    #21266

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Ufd1 (Ufd1l Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-pro-sumo2

    Plasmid

    #25102

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO2 (SUMO2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET29b-Ava3858-3856

    Plasmid

    #45745

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3858-3856
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-iLOV-Rsn2

    Plasmid

    #61687

    Purpose

    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV

    Depositor

    Brian Smith

    Insert

    Ranaspumin-2
    

    Tags

    His and iLOV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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