We narrowed to 59,329 results for: cloning

1. pHMR1A

   Plasmid

   #102957

   Purpose

   gene cloning of MR1A

   Depositor

   Lucia Mori, Gennaro De Libero

   Article

   Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

   Insert

   MR1A cDNA (MR1 Human)
   

   Use

   Cloning

   Mutation

   Stop codon has been removed by HindIII site

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p5E-EF1a (JDW 1164)

   Plasmid

   #224473

   Purpose

   Gateway compatible 5' entry clone containing the human EF1a promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EF1a promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p5E-hs_ICAM_full (JDW 1047)

   Plasmid

   #224479

   Purpose

   Gateway compatible 5' entry clone containing the Human ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   ICAM2 promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)

   Plasmid

   #242572

   Purpose

   Gateway 3' entry clone containing an IRES Luciferase

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-Luciferase-WPRE-bGH-pA
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pME-LiCre (JDW 1119)

   Plasmid

   #242560

   Purpose

   Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LiCre (AsLOV2-CreE340AD341A)
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3E-mCherry-SV40-pA (JDW 1417)

   Plasmid

   #242570

   Purpose

   Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCherry stop SV40 pA
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMEF2c-AHF-DreERT2 (JDW 196)

   Plasmid

   #242586

   Purpose

   The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DreERT2
   

   Expression

   Mammalian

   Promoter

   mouse Mef2c

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

   Plasmid

   #242589

   Purpose

   A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh (actin nanobody)
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

   Plasmid

   #229827

   Purpose

   A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xnls-TagBFP2-MCS-FLAG
   

   Expression

   Mammalian

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB-Puro-DEST (JDW 925)

    Plasmid

    #229808

    Purpose

    A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

    Plasmid

    #229811

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA transactivator-LexA-Operon-nls-GFP
    

    Use

    Tol2 based expression vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

    Plasmid

    #229823

    Purpose

    A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p5E-hs_Cldn5 (JDW 1120)

    Plasmid

    #224481

    Purpose

    Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Claudin-5 promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCoofy57

    Plasmid

    #122009

    Purpose

    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His8 and Multi1: depending on the LP2 primer, eit…

    Expression

    Mammalian

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCoofy55

    Plasmid

    #122007

    Purpose

    Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His10-SumoStar (Life Sensors) and Multi2: dependi…

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C413-E45: attB4-hr5-ie1p>-attB1r

    Plasmid

    #162930

    Purpose

    Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV ie1 promoter with hr5 enhancer
    

    Use

    Synthetic Biology

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCoofy64

    Plasmid

    #122016

    Purpose

    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Insect

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBJT001

    Plasmid

    #225152

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge