We narrowed to 21,643 results for: ESC;

1. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1.1-eGFP

   Plasmid

   #162738

   Purpose

   Fluorescent reporter for CHO cells expression studies

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean3-LaminB1-10

   Plasmid

   #55425

   Purpose

   Localization: Nuclear Envelope, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pBAD-R-iLACCO1.1

   Plasmid

   #208020

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCardinal-NUP50-N-10

   Plasmid

   #56170

   Purpose

   Localization: Nuclear Pore, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   NUP50 (NUP50 Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

   Plasmid

   #70191

   Purpose

   Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   ClpX (clpX )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_mGFP-FGFR3-mScarlet

   Plasmid

   #192509

   Purpose

   Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc-mScarlet
   

   Use

   Tags

   mGFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV iGluf

   Plasmid

   #105833

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR E25D variant
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Glu 25 to Asp

   Promoter

   CMV

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-rtTA-zeo

   Plasmid

   #183233

   Purpose

   Lentiviral vector expressing the rtTa gene

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   rtTA-T2A-Zeo
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mIFP-Nup50-N-10

    Plasmid

    #56233

    Purpose

    Localization: Nuclear Pore, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Nup50 (NUP50 Human)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pUASTattB_NSnoFbox-vhhGFP4

    Plasmid

    #35578

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F box domain of slmb deleted.

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLENTI_RPS3_K214R

    Plasmid

    #127132

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3 (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K214R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEX- HcRed -hLC3ΔG

    Plasmid

    #24992

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pBabe(puro) mouse Egr4

    Plasmid

    #108105

    Purpose

    Retroviral vector for expression of mouse Egr4

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    Egr4 (Egr4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cx43-sfGFP

    Plasmid

    #70053

    Purpose

    Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    non-monomerized superfolder GFP (V207)

    Expression

    Mutation

    fused in frame to non-monomerized superfolder GFP

    Promoter

    CMV

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mRuby2-H2B-6

    Plasmid

    #55897

    Purpose

    Localization: Nucleus/Histones, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mOrange2-Clathrin-15

    Plasmid

    #57947

    Purpose

    Localization: Clathrin Vesicles, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only