Purpose
PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
Inserts
Pyrrolysyl-tRNA Synthetase (2 copies) (
pylS Methanosarcina mazei)
tRNA Pyl
Promoter
araBAD, rrnB, and glnS and proK
Available Since
Jan. 22, 2025
Availability
Academic Institutions and Nonprofits only