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lentiSAMv2 C2CD4A-TSS-guide2

Plasmid

#125481

Purpose

CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 VPS13C-TSS-guide2

Plasmid

#125477

Purpose

CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 VPS13C-TSS-guide1

Plasmid

#125476

Purpose

CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A/B-enh3-guide3

Plasmid

#125475

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A/B-enh2-guide2

Plasmid

#125470

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A/B-enh1-guide3

Plasmid

#125467

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 TCF7L2 TSS-guide4

Plasmid

#118187

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 TCF7L2 TSS-guide1

Plasmid

#118184

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 OPTN TSS-guide4

Plasmid

#118199

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 TCF7L2 TSS-guide3

Plasmid

#118186

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgRBFOX1-2098

Plasmid

#115452

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2098 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgRBFOX1-2083

Plasmid

#115451

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2083 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

Plasmid

#108482

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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hFlt1 V767A

Plasmid

#86038

Purpose

human VEGFR1 with V to A mutation at position 767

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 V767A (FLT1 Human)

Tags

FLAG, HA (please see depositor comments below), a…

Expression

Mammalian

Mutation

V767A

Promoter

CMV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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LacI-11/109-pHG165c

Plasmid

#90059

Purpose

Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI-11/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1 ObLiGaRe Donor vector/EPB58

Plasmid

#90016

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by inverted ZFN binding sites (PPP1R12C Human)

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p5M-PR-B-1-688

Plasmid

#89115

Purpose

mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor-B N-term+DNA binding domain (PGR Human)

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker, contai…

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5 713

Plasmid

#65246

Purpose

Expression of HA tagged ERK5 AA 2-713 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 713 (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 714-817

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
PGL3-Ogt (-178 to +233 bp)

Plasmid

#79266

Purpose

To identify the Cis and Trans acting elements that control Ogt gene transcription.

Depositor

Steven Jones

Article

Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

Insert

Ogt promoter element (Ogt Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only