We narrowed to 23,196 results for: ESC

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pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

Plasmid

#70191

Purpose

Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

ClpX (clpX )

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLifeact-mScarlet3-H (mYongHong)

Plasmid

#239415

Purpose

Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Lifeact-mScarlet3-H

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP - FLAG-APEX2- hs Lamin B1

Plasmid

#139442

Purpose

APEX2-Lamin B1 expression plasmid

Depositor

Yixian Zheng

Article

Tran et al bioRxiv 2020.02.05.935635

Insert

LMNB1 (LMNB1 Human)

Tags

3xGGGGS linker (between APEX2 and Lamin B1), APEX…

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Actin Binding Domain Tension Sensor

Plasmid

#215368

Purpose

Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

Depositor

Brenton Hoffman

Article

Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

Actin Binding Domain Tension Sensor

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL_PylRS(WT)-tRNA

Plasmid

#223512

Purpose

PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Inserts

Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
tRNA Pyl

Tags

6xHis

Expression

Bacterial

Promoter

araBAD, rrnB, and glnS and proK

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-TfR-mApple

Plasmid

#179384

Purpose

Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

Transferrin receptor 1 - mApple (TFRC Synthetic, Human)

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMV_AA050

Plasmid

#216107

Purpose

gDNA expression for Cas9 with ChenF+E trRNA (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gDNA expression for Cas9 with ChenF+E trRNA

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#232754

Purpose

Expresses EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

EGFP

Use

Lentiviral

Promoter

EF1a

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS

Plasmid

#86677

Purpose

Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

EGFP-NLS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6s.WPRE.SV40

Plasmid

#100844

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR4_G3BP2

Plasmid

#127105

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP2 (G3BP2 Human)

Use

Gateway shuttling vector

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPGK-SB13

Plasmid

#236078

Purpose

SB13 transposase, for use with Sleeping Beauty transponson plasmids

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Insert

SB13 transposase

Use

Sleeping beauty (sb) transposase plasmid

Promoter

PGK

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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Lyn FAK biosensor

Plasmid

#78299

Purpose

FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Lyn Fak biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-ExRai-AktAR2

Plasmid

#184047

Purpose

Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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