164,826 results

1. GATA3-pCDNA3.1

   Plasmid

   #115271

   Purpose

   Expresses human GATA3 in mammalian cells

   Depositor

   Arne S. Schaefer

   Insert

   GATA3 (GATA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCH67 (crRNA expression vector)

   Plasmid

   #217337

   Purpose

   CROP-seq crRNA expression vector with GFP and puro marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-Oct3/4

   Plasmid

   #13366

   Purpose

   Retroviral expression of mouse Oct3/4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-BRAF-V600E

   Plasmid

   #116204

   Purpose

   Lentiviral expression of BRAF V600E

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   V600E

   Promoter

   Available Since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMPRA2

   Plasmid

   #49350

   Purpose

   Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiV_Neo

   Plasmid

   #108101

   Purpose

   Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSXneo 135(T2AG3)

   Plasmid

   #12402

   Depositor

   Titia de Lange

   Article

   Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

   Insert

   135(T2AG3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SMVP-Cas9C

   Plasmid

   #80931

   Purpose

   Expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pet28a_Human_H3.3

    Plasmid

    #42632

    Depositor

    Joe Landry

    Insert

    Human H3.3 (H3-3A Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. FH1-MGT#2-mCherry

    Plasmid

    #164097

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX - T7pro - EBFP

    Plasmid

    #138615

    Purpose

    Express EBFP with an upstream T7 promoter in a lentiviral backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. GLP1R-Tango

    Plasmid

    #66295

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3XFlag DVL2 (WT)

    Plasmid

    #24802

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEGFP-N1 human cofilin S3E

    Plasmid

    #50861

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-Brainbow-2.1 R

    Plasmid

    #18723

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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