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pRoLuc

Plasmid

#135284

Purpose

GBPart - RoLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

R. ohbai Luciferase

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M47)

Plasmid

#23286

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax(M47)

Expression

Mammalian

Mutation

L319R L320S

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FLII12Pglu-700uDelta6

Plasmid

#13568

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 6aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pKM808

Plasmid

#134181

Purpose

Lentiviral sgRNA plasmid with blasticidin resistance

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_RBFOX2-Halo

Plasmid

#175304

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

RBFOX2 (RBFOX2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par6C

Plasmid

#15474

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6C (PARD6A Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pmScarlet-H_NES_C1

Plasmid

#85061

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs N-terminal sgRNA

Plasmid

#207093

Purpose

pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGCGGGACTCGGCGGCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)1

Plasmid

#127089

Purpose

Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP human SUMO3 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_TXN_Thioredoxin

Plasmid

#109778

Purpose

Protein expression and purification of TXN_Thioredoxin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TXN_Thioredoxin (TXN Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP3-Halo

Plasmid

#175310

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP3 (IGF2BP3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HA-DN-14-3-3 zeta (R56A/R60A)

Plasmid

#116889

Purpose

Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR

Plasmid

#208328

Purpose

MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

H2B-MiniR

Tags

MiniR

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-CRISPR

Plasmid

#164797

Purpose

For gene editing in oomycetes

Depositor

Miaoying Tian

Article

Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

Inserts

PsNLS-hSpCas9
sgRNA expression cassette

Use

Oomycete expression

Promoter

HAM34 and RPL41

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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VRER-ABEmax

Plasmid

#119812

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI823

Plasmid

#216632

Purpose

Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID (split site variant)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pOPS0377

Plasmid

#115509

Purpose

Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Constitutive promoter pNeo pOGG001

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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