179,359 results
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Plasmid#75140PurposeExpresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 91-710PromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CMV-DIO-Magneto2.0-sNRPpA
Plasmid#74307PurposeCre-dependent expression of Magneto2.0 in mammalian cellsDepositorUseAAV and Cre/LoxTagsFlagExpressionMammalianMutationTRPV4: delta 760-871PromoterCMV (after Cre recombination)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-ninein
Plasmid#73519PurposeEncodes mouse ninein tagged with an EGFP tag at its N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertninein (Nin Mouse)
TagsEGFP tagExpressionMammalianPromoterCMVAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR3.1-mIL2
Plasmid#74467Purposeexpression of mouse IL2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIL2 (Il2 Mouse)
ExpressionMammalianPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-SLC38A9.1
Plasmid#71855PurposeoverexpressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
TagsFLAGExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZW1-FCS-circRNA
Plasmid#73449PurposeExpression of circular RNADepositorAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIP-VEGFA121
Plasmid#73017PurposeRetroviral expression of human VEGFA121DepositorAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseGateway CloningAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GRM2-Tango
Plasmid#66388PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRM2 (GRM2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CXCR7-Tango
Plasmid#66265PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR7 (ACKR3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9/pTREX-n
Plasmid#68708PurposeExpresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
UseCRISPRTagsFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …MutationC4239T mutation that eliminates a BamHI restricti…Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJH46
Plasmid#67272PurposeConjugally transferable red recombinogenic plasmidDepositorInserttraJ, cat, oriT
UseRed recombinasePromotercat gene promoter from PGNS-BACAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Prig-3::CD4::spGFP1-10
Plasmid#65829PurposeFor membrane contactDepositorPublicationInsertHomo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
TagsspGFP1-10ExpressionWormPromoterPrig-3Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRC_RR_VP1_r1c3
Plasmid#65724PurposeRescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSRDepositorInsertmCherry
UseAAVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERoxBFP
Plasmid#68126Purposemammalian expression of ER localized oxBFPDepositorInsertoxBFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU6a/LacZ
Plasmid#66195Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6aAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by