We narrowed to 24,946 results for: CHI;

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pKK233-hCaM

Plasmid

#47598

Depositor

Emanuel Strehler

Article

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CaM (CALM3 Human)

Expression

Bacterial

Mutation

contains 469 bp fragment (nt 92-559)

Promoter

trc

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

Plasmid

#213402

Purpose

douple floxed, myristoylation (myr) site for membrane targeting and EGFP

Depositor

Katrin Willig

Article

Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

Insert

EGFP

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shWT1_B

Plasmid

#217637

Purpose

Dox inducible shRNA targeting WT-1

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shWT1_B (WT1 Human)

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBS FGF3 isoform 1

Plasmid

#19230

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
gRNA NIA Null

Plasmid

#198728

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM-RFP

Plasmid

#207651

Purpose

Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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NSSCP

Plasmid

#183190

Purpose

mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

Depositor

Hsiao-Chun Huang

Article

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

mRFP-PopZ
DivIVA-GFP(tevR-50)
SpmX-TEV(C)
TEV(N)-SpmX

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP30_MARV

Plasmid

#135472

Purpose

Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

VP30

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAGD236

Plasmid

#219497

Purpose

pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

sfGFP, Eco1 msr, Eco1 RT

Expression

Bacterial

Promoter

T7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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EGR-1 TurboRFP

Plasmid

#52306

Purpose

Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Article

Varma et al Lab Chip. 2015 Feb 4.

Insert

EGR-1 Promoter (Egr1 Mouse)

Tags

TurboRFP

Expression

Mammalian

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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p40phox-PX (1-144)

Plasmid

#119123

Purpose

Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p40phox-PX (1-144)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas12a

Plasmid

#158713

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mECitrine

Plasmid

#105151

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

kanMX6-P3nmt1-mECitrine

Tags

3nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

3nmt1

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pKL001

Plasmid

#98917

Purpose

Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

CaPR

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ALK4 T206D

Plasmid

#80880

Purpose

mammalian expression of ALK4 T206D

Depositor

Aristidis Moustakas, Peter ten Dijke

Article

ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

Insert

ALK4 (ACVR1B Human)

Tags

HA

Expression

Mammalian

Mutation

T206D

Promoter

CMV

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pWPI/hPLK2WT/Neo

Plasmid

#35385

Depositor

Anurag Tandon

Article

Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

Insert

hPLK2 (PLK2 Human)

Use

Lentiviral

Tags

EMCV IRES-Neomycin cassette and V5 epitope and Po…

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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