We narrowed to 19,085 results for: REV;

Showing: 11101 - 11120 of 19085 results

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pDONR223 GABARAPL1 WT

Plasmid

#123083

Purpose

Gateway entry clone encoding human GABARAPL1 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-NL1

Plasmid

#173007

Purpose

For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-NL1

Tags

DHFR and EGFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF3

Plasmid

#207815

Purpose

N-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF3 (NFE2L3 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF3-V5-miniTurbo

Plasmid

#207816

Purpose

C-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF3-V5-miniTurbo (NFE2L3 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJR021

Plasmid

#198814

Purpose

Variant of yellow fluorescent protein for transient gene expression

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::destabilizedYFP::let-838 3'UTR

Expression

Worm

Mutation

YFP with destabilized signal from mouse ornithine…

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-PH-SWAP70

Plasmid

#84582

Purpose

Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 (SWAP70 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pBa-FRB-3myc-Rab5a

Plasmid

#64209

Purpose

Rab5a with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab5a (Rab5a Mouse)

Tags

3myc and FRB

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgHK2_2

Plasmid

#163461

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Sema4g-Fc-His

Plasmid

#72160

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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cpGFP-Adhiron_flex

Plasmid

#165044

Purpose

Flexible linker control for POLArISact expression in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

cpGFP-Adhiron (connected with a flexible linker)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_4

Plasmid

#103922

Purpose

Protein expression and purification of PTN13 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CHEK2 C3.2 gRNA

Plasmid

#90628

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CHEK2 (Guide Designation C3.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_Actb sgRNA / hSpCas9

Plasmid

#172832

Purpose

Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(L)-Fc-His

Plasmid

#72151

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Golgi-7

Plasmid

#55420

Purpose

Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Golgi (B4GALT1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_SKAP2-1/1

Plasmid

#91447

Purpose

Protein expression and purification of human SH3 domain construct SKAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SKAP2-1/1 (SKAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PARD3_PDZ_3

Plasmid

#103959

Purpose

Protein expression and purification of PAR3 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PARD3 (PARD3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-CCCAP

Plasmid

#136798

Purpose

Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CCCAP (SDCCAG8 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-mTDGa.0

Plasmid

#81050

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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