We narrowed to 11,189 results for: cat.1

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CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

Plasmid

#91820

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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T7-VEE-OKS-iG

Plasmid

#58974

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
GLIS (GLIS1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-OKS-iG

Plasmid

#58975

Purpose

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Inserts

Oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
GLIS (GLIS1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Tags

N-terminal mScarlet tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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TRCN0000072213

Plasmid

#78159

Purpose

shRFP control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

RFP

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pTwist-SARS-CoV-2 Δ18 B.1.351v2

Plasmid

#169463

Purpose

Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.1.7

Plasmid

#169459

Purpose

Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v3

Plasmid

#169464

Purpose

Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v1

Plasmid

#169462

Purpose

Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.429

Plasmid

#169461

Purpose

Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.429 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.1.298

Plasmid

#169460

Purpose

Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 P.2/B.1.1.248

Plasmid

#173477

Purpose

Encodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 Spike Truncated to remove 18 amino acids

Expression

Mammalian

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL-CS

Plasmid

#187713

Purpose

Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_1

Plasmid

#195130

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 1 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcrDNA3.1-PRKACA

Plasmid

#100890

Purpose

Mammalian expression of PRKACA

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)

Expression

Mammalian

Mutation

Isoform 1 wild type

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2

Plasmid

#72207

Purpose

Mammalian expression of full-length human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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mEtfdh CRISPR_1

Plasmid

#232312

Purpose

Mouse Etfdh Gene Knockout (gRNA 1)

Depositor

Ivan Topisirovic

Article

Papadopoli et al bioRxiv 2024.10.25.620155

Insert

Etfdh-targeting gRNA (Etfdh Mouse)

Use

Lentiviral

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

Plasmid

#169469

Purpose

Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEUT RUVBL1 Delete

Plasmid

#159144

Purpose

Expression and protien purification of mutated RUVBL1

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL1 (RUVBL1 Human)

Expression

Bacterial

Mutation

amino acid 1-463, Glu134-Glu237 mutated to GPPG

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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