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pAB170

Plasmid

#101669

Purpose

Opto-T7RNAP*(69), half expr.

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_002

Plasmid

#180527

Purpose

Entry vector containing J23104 promoter

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23104 promoter

Expression

Bacterial

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC005

Plasmid

#171140

Purpose

For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

XylS-Pm

Use

CRISPR

Expression

Bacterial

Promoter

Pm

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAdh 3'UTR Short

Plasmid

#165800

Purpose

Drosophila HSP70B 3'UTR sequence

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70 3'UTR

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-UAS

Plasmid

#119001

Purpose

Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Expression

Bacterial

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSR305

Plasmid

#124712

Purpose

Expression of uhpB under Ptac

Depositor

Jeffrey Tabor

Publication

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpB (uhpB )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE

Plasmid

#113853

Purpose

Superfolder GFP and tdTomato linked by T2A (V linker)

Depositor

Mario Capecchi

Insert

sfGFP-T2A-tdTomato

Expression

Mammalian

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-NS

Plasmid

#80497

Purpose

Multisite gateway entry clone for adding N-terminal fusions of mVenus

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

mVenus (no STOP)

Use

Multisite gateway middle entry clone

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_J23100

Plasmid

#74097

Purpose

BBa_J23100 promoter (iGEM collection)

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_J23100 constitutive promoter (Sigma70)

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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jk57

Plasmid

#87010

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Publication

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

Nlg1AB
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-FRB-HA no-pA

Plasmid

#82595

Purpose

3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-FRB-HA (no pA)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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PP275

Plasmid

#78968

Purpose

Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c-PK12

Plasmid

#78371

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

12xPK
natMX6

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c-PK6

Plasmid

#78369

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
natMX6

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ03

Plasmid

#82399

Purpose

pACSA_J23116_tetR PEZ1_gfp

Depositor

Brian Pfleger

Publication

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ1

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhab-3'UTR

Plasmid

#61782

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR

Use

Luciferase

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR

Plasmid

#61779

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR

Use

Luciferase

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR

Plasmid

#61778

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR

Use

Luciferase

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only