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Flag‐mTim3 (T2 truncation)

Plasmid

#49211

Purpose

expresses Flag tagged mouse Tim3 (T2 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T2 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T2 truncation ( lacks all but one tyrosine, which…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutNRF-1(-41)-like

Plasmid

#66744

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGC (-41) to ATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/dmutNRF-1(-41;-35)-like

Plasmid

#66741

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCAGGCGC (-41;-35) to ATTAGGATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-WDR12-S127F

Plasmid

#116707

Purpose

Lentiviral expression of WDR12 S127F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WDR12 (WDR12 Human)

Use

Lentiviral

Mutation

S127F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ROR1-T69M

Plasmid

#116675

Purpose

Lentiviral expression of ROR1 T69M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ROR1 (ROR1 Human)

Use

Lentiviral

Mutation

T69M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TLP-bio

Plasmid

#47787

Purpose

Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised TLP

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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PF3D7_1431400-bio

Plasmid

#47791

Purpose

Expresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised PF3D7_1431400

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR Full mut

Plasmid

#32489

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated in the entire miR-210 binding region

Promoter

CMV

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only
PLCG2-P522R

Plasmid

#254918

Purpose

Express PLCG2-P522R (protective variant) in human induced pluripotent stem cells.

Depositor

Jason Meyer

Article

Bedford et al Alzheimers Dement. 2025 Oct;21(10):e7077

Insert

phospholipase C gamma 2 - P522R (PLCG2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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PLCG2-M28L

Plasmid

#254917

Purpose

Express PLCG2-M28L (disease variant) in human induced pluripotent stem cells.

Depositor

Jason Meyer

Article

Bedford et al Alzheimers Dement. 2025 Oct;21(10):e7077

Insert

phospholipase C gamma 2 - M28L (PLCG2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSBtet-GP-α7-NACHO_EM

Plasmid

#253979

Purpose

Expresses human alpha7 nicotinic acetylcholine receptor and nAChO in bicistronic system

Depositor

Ryan Hibbs

Article

Burke et al Cell. 2024 Feb 29;187(5):1160-1176.e21.

Insert

Human alpha7 nicotinic acetylcholine receptor (CHRNA7 Human)

Tags

Twin strep tag

Expression

Mammalian

Mutation

Deletion in M3M4 loop with M3M4 loop insertion of…

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-NRF1-FL

Plasmid

#237453

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full-length NRF1 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 full length

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-TAZ-CAMTA1

Plasmid

#235679

Purpose

Express TAZ-CAMTA1 fusion gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

Insert

TAZ-CAMTA1 (WWTR1, CAMTA1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGH020 sgZmat3.1

Plasmid

#227943

Purpose

sgRNA targeting Zmat3, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Zmat3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGH020 sgZmat3.2

Plasmid

#227944

Purpose

sgRNA targeting Zmat3, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Zmat3.2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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