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pF(UG) hSyn NS34a-msGFP-'S-mito

Plasmid

#158768

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Publication

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE5

Plasmid

#169267

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E5 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE8

Plasmid

#169268

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E8 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 G>T minigene

Plasmid

#169272

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 G>T minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(946G>T)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

Plasmid

#169273

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 Exon1-6 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Lckg2a,Y394F,Y505F)

Plasmid

#177894

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Publication

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F,Y505F

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala

Plasmid

#179716

Purpose

Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pCMV-KIF5C(1-560)-SNAP

Plasmid

#261906

Purpose

Truncated constitutively active kinesin-1 motor (KIF5C residues 1-560) fused to SNAP, used as a marker of nascent axons in cultured neurons.

Depositor

Frank Bradke

Publication

Lin et al Nature. 2026 Jul 8. doi: 10.1038/s41586-

Insert

Kif5c (Kif5c Rat)

Tags

SNAP-tag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Oct. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pBR189

Plasmid

#259200

Purpose

Constitutively expresses eNRGies biosensor with FXa-site and mEGFP (ECD) and FusionRed (ICD)

Depositor

Beatrice Ramm, Jared Toettcher

Publication

Ramm et al bioRxiv 2026.06.19.733436

Inserts

mEGFP
FusionRed

Tags

20 AA GS linker, CD4 signal sequence (mouse) - G4…

Expression

Mammalian

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBR198

Plasmid

#259201

Purpose

Constitutively expresses eNRGies biosensor with MMP-9-site and mEGFP (ECD) and FusionRed (ICD)

Depositor

Beatrice Ramm, Jared Toettcher

Publication

Ramm et al bioRxiv 2026.06.19.733436

Inserts

mEGFP
FusionRed

Tags

20 AA GS linker, CD4 signal sequence (mouse) - G4…

Expression

Mammalian

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBR235

Plasmid

#259203

Purpose

Constitutively expresses ADAM17 eNRGies biosensor with TGFa-site and mEGFP (ECD) and FusionRed (ICD)

Depositor

Beatrice Ramm, Jared Toettcher

Publication

Ramm et al bioRxiv 2026.06.19.733436

Inserts

mEGFP
FusionRed

Tags

10 AA GS linker, CD4 signal sequence (mouse) - G4…

Expression

Mammalian

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBR188

Plasmid

#259199

Purpose

Constitutively expresses eNRGies biosensor with EK-site and mEGFP (ECD) and FusionRed (ICD)

Depositor

Beatrice Ramm, Jared Toettcher

Publication

Ramm et al bioRxiv 2026.06.19.733436

Inserts

mEGFP
FusionRed

Tags

20 AA GS linker, CD4 signal sequence (mouse) - G4…

Expression

Mammalian

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 K289A/R293A/R301A

Plasmid

#250572

Purpose

Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 K289A/R293A/R301A in mammalian cells

Depositor

Allison Didychuk

Publication

Bailey et al PLoS Pathog. 2026 Sep 10;22(9):e1014540.

Insert

UL32

Tags

TwinStrepTagII

Expression

Mammalian

Mutation

Amino acids K289, R293, R301 mutated to alanine

Promoter

CMV

Available since

Oct. 1, 2026

Availability

Academic Institutions and Nonprofits only

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HA-RELT deletion I

Plasmid

#260227

Purpose

Expression in mammalian cells

Depositor

John Cusick

Publication

Moua et al Biochem Biophys Res Commun. 2017 Sep 9;4

Insert

RELT (Receptor Expressed in Lymphoid Tissues) (RELT Human)

Tags

None

Expression

Mammalian

Mutation

Lacking 110 amino acids from C-terminal end (1-32…

Available since

Sept. 23, 2026

Availability

Academic Institutions and Nonprofits only

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HA-RELT deletion H

Plasmid

#260226

Purpose

Expression in mammalian cells

Depositor

John Cusick

Publication

Moua et al Biochem Biophys Res Commun. 2017 Sep 9;4

Insert

RELT (Receptor Expressed in Lymphoid Tissues) (RELT Human)

Tags

None

Expression

Mammalian

Mutation

Lacking 178 amino acids from C-terminal end (1-25…

Available since

Sept. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-tev-4xTS

Plasmid

#247150

Purpose

Nanobody expression

Depositor

Dominik Buser

Publication

Buser et al FEBS Lett. 2026 Aug 15. doi: 10.1002/187

Insert

VHH-tev-4xTS

Tags

BAP epitope, HA-tag, His6, and T7-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-TGN46

Plasmid

#247140

Purpose

EGFP reporter plasmid

Depositor

Dominik Buser

Publication

Buser et al FEBS Lett. 2026 Aug 15. doi: 10.1002/187

Insert

TGN46 (TGOLN2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-VNp6-mCherry2_OmpX-mNeongreen

Plasmid

#245926

Purpose

Co-expression of VNp6-mCherry2 and OmpX-mNeongreen fusions in E. coli cells

Depositor

Dan Mulvihill

Publication

Streather et al J Extracell Vesicles. 2026 Aug;15(8):e70

Inserts

VNp6-mCherry2
OmpX-mNeongreen

Tags

His6, mCherry2, and mNeongreen

Expression

Bacterial

Promoter

T7

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-ssDsbA-mCherry2 VNp6-mNeongreen

Plasmid

#245929

Purpose

Co-expression of ssDsbA-mCherry2 and VNp6-mNeongreen fusions in E. coli cells

Depositor

Dan Mulvihill

Publication

Streather et al J Extracell Vesicles. 2026 Aug;15(8):e70

Inserts

ssDsbA-mCherry2
VNp6-mNeongreen

Tags

mCherry2 and mNeongreen

Expression

Bacterial

Promoter

T7

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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