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pF3BGX-T2A-GAL80

Plasmid

#138396

Purpose

RMCE vector for generating C-terminal T2A-GAL80 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL80

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pKM568

Plasmid

#134217

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEYC1A

Plasmid

#127758

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in convergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0511

Plasmid

#124002

Purpose

Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_phubc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0512

Plasmid

#124003

Purpose

Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_049

Plasmid

#123946

Purpose

Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hThal5.10-2_hg18 landing pad destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTK729

Plasmid

#114710

Purpose

Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705+1700-2115ΔNLS)-RFP-Nano

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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T7-AglK-His

Plasmid

#89714

Purpose

Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglK

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK388

Plasmid

#114713

Purpose

mAID-mClover-3FLAG tagging at DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRC02

Plasmid

#114681

Purpose

sfGFP.Strand10-Haloenzyme

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP.Strand10-Haloenzyme

Tags

His10

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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POT2-CYC1p-ycrtE-ADH1t

Plasmid

#65354

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-CYC1p-ycrtI-ADH1t

Plasmid

#65357

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TEF2

Plasmid

#65337

Purpose

construction of TEF2 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 promoter

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSθ

Plasmid

#68289

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP30

Plasmid

#65499

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-mCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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