2,294 results

1. p045

   Plasmid

   #70057

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with pDC184

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   C2AB-linker-SNAP-25_C
   SNAP-25_N
   

   Tags

   No tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Myo9b R1695M

   Plasmid

   #134911

   Purpose

   Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

   Depositor

   Martin Bähler

   Article

   Muller et al Mol Biol Cell. 1997 Oct;8(10):2039-53. d

   Insert

   Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed arginine 1695 to methionine

   Promoter

   CMV IE

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mIFP-Lysosomes-20

   Plasmid

   #56228

   Purpose

   Localization: Lysosome Membrane, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LAMP1
   

   Tags

   mIFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. AAV-SYN_mEGFP-CREB (S133A)

   Plasmid

   #137010

   Purpose

   AAV backbone, mEGFP-CREB donor for G-CREB with S133A mutation

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   AAV

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Changes Serine 133 into Alanine

   Promoter

   hSynapsin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS426-GFP-PLCdelta-PH

   Plasmid

   #71375

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   PLCdelta-PH
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pECFP-C1-rGAT

   Plasmid

   #70108

   Purpose

   mammalian expression of rat GAT with N terminal CFP fusion

   Depositor

   Harald Sitte

   Article

   Scholze et al J Biol Chem. 2002 Nov 15;277(46):43682-9

   Insert

   GAT (Slc6a1 Rat)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIS-krPAM2

   Plasmid

   #25457

   Depositor

   Richard Mains

   Article

   Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

   Insert

   PAM2 (Pam Rat)
   

   Expression

   Mammalian

   Mutation

   Exon A deleted (exon A extends from nt 1475 to n…

   Available Since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

8. RAT

   Plasmid

   #187416

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Expression

   Insect

   Promoter

   PH and p10

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RSV CREB

   Plasmid

   #22394

   Depositor

   Marc Montminy

   Article

   Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

   Insert

   CREB (Creb1 Rat)
   

   Expression

   Mammalian

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pLenLox_U6 Nlgn2

    Plasmid

    #59358

    Purpose

    Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

    Depositor

    Peter Scheiffele

    Article

    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

    Inserts

    Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Mouse, Rat)
    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and mouse U6

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5-ratERK2-Q103A

    Plasmid

    #40816

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Q103A

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSIL-eGFP-shACTN3

    Plasmid

    #52678

    Purpose

    shRNA against α-actinin-3 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN3 (Actn3 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Promoter

    U6

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dronpa-GluR2

    Plasmid

    #62735

    Purpose

    Excitation:503, Emission:518

    Depositor

    Michael Davidson

    Insert

    GluR2
    

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pCI-neo-myc-LC3(G120A)-HA

    Plasmid

    #45245

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Glycine at position 120 mutated to Alanine

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pR008_APAD

    Plasmid

    #204818

    Purpose

    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    

    Tags

    HA and Flag and huADAR2dd

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIC-myc-GBAi

    Plasmid

    #171753

    Purpose

    For the mammalian expression of myc-GBAi

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Nov. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pEF Doc2alpha WT GFP

    Plasmid

    #128819

    Purpose

    To visualize Doc2alpha WT in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

19. GSE22 T329A in pENTR1A (w187-1)

    Plasmid

    #22253

    Depositor

    Eric Campeau

    Insert

    Genetic suppressor element #22, T329A
    

    Use

    Entry vector

    Mutation

    Threonine 329 is mutated to Alanine (p53 nomencat…

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. LUC45 (L/Sec/mutP)

    Plasmid

    #112148

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U) and SE…

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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